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dc.contributor.author Bruzzone, Ariana
dc.contributor.author Vanzulli, Silvia
dc.contributor.author Soldati, Rocío Cinthia
dc.contributor.author Giulianelli, Sebastian Jesus
dc.contributor.author Lanari, Claudia Lee Malvina
dc.contributor.author Luthy, Isabel Alicia
dc.date.available 2017-09-18T18:40:50Z
dc.date.issued 2009-02
dc.identifier.citation Bruzzone, Ariana; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian Jesus; Lanari, Claudia Lee Malvina; et al.; Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice; Wiley; Journal of Cellular Physiology; 219; 2; 2-2009; 477-484
dc.identifier.issn 0021-9541
dc.identifier.uri http://hdl.handle.net/11336/24475
dc.description.abstract Breast cancer is the most frequent cancer in women. However, in vivo hormone receptor positive and metas­tatic models are scarce. The aim of the pre­­sent manus­cript was to assess if the novel steroid receptor positive hu­man cell li­nes IBH-4, IBH-6 and IBH-7 developed in our laboratory from primary in­fil­trant duc­tal carcinomas are good mo­dels to study in vivo human breast cancer. Cell lines or tumors were inoculated to nude mice in the presence or ab­­sence of hormone supplementation. Growth was analyzed by ANOVA follo­wed by Tukey-Kra­mer´s test. Steroid hormone expression was assessed by con­focal immuno­fluo­res­cen­ce and Western blotting. The histology of the tu­mors was ana­lyzed. IBH-4 and IBH-6 cells were ino­­­cu­lated to nude mice and 100% of the in­jected mi­ce deve­loped tu­mors in the presence or absence of hor­mo­­ne treat­ment, al­though tamo­xifen inhibited growth. IBH-4 and IBH-6 cell lines in vivo gave rise to poorly diffe­­­ren­tiated carci­no­mas with areas of solid growth and sar­co­­ma­toid areas showing no mor­pho­lo­gical signs of epithe­lial differentiation. Distinct fea­tu­res of ma­lignancy were ob­served. IBH-7 tu­mors in animals receiving estradiol were semi-diffe­ren­­tia­ted ade­no­carcinomas. IBH-7 cells grew only in the pre­­sen­ce of estradiol, but even with hormone addition, the tumor take was 20%. The­se tu­mors me­tas­ta­sized to the uterus and lung and vascular tumor emboli we­re evi­dent. IBH-7 tu­mors were invasive and able to break through the pe­ri­­to­neum. As a conclusion, IBH-4 and IBH-6 are good models for studying tu­mor pro­­gres­sion, whereas IBH-7 is a good model for tumor take, being metastatic and stric­tly estro­gen-dependent.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject BREAST CANCER
dc.subject HUMAN CELL LINES
dc.subject HORMONE RECEPTORS
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.subject.classification Patología
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-09-13T16:15:16Z
dc.identifier.eissn 1097-4652
dc.journal.volume 219
dc.journal.number 2
dc.journal.pagination 477-484
dc.journal.pais Estados Unidos
dc.journal.ciudad Hoboken
dc.description.fil Fil: Bruzzone, Ariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Vanzulli, Silvia. Academia Nacional de Medicina de Buenos Aires; Argentina
dc.description.fil Fil: Soldati, Rocío Cinthia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Giulianelli, Sebastian Jesus. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Lanari, Claudia Lee Malvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil Fil: Luthy, Isabel Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title Journal of Cellular Physiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/jcp.21694
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/jcp.21694/abstract


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)