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Artículo

Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice

Bruzzone, ArianaIcon ; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian JesusIcon ; Lanari, Claudia Lee MalvinaIcon ; Luthy, Isabel AliciaIcon
Fecha de publicación: 02/2009
Editorial: Wiley
Revista: Journal of Cellular Physiology
ISSN: 0021-9541
e-ISSN: 1097-4652
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular; Patología

Resumen

Breast cancer is the most frequent cancer in women. However, in vivo hormone receptor positive and metas­tatic models are scarce. The aim of the pre­­sent manus­cript was to assess if the novel steroid receptor positive hu­man cell li­nes IBH-4, IBH-6 and IBH-7 developed in our laboratory from primary in­fil­trant duc­tal carcinomas are good mo­dels to study in vivo human breast cancer. Cell lines or tumors were inoculated to nude mice in the presence or ab­­sence of hormone supplementation. Growth was analyzed by ANOVA follo­wed by Tukey-Kra­mer´s test. Steroid hormone expression was assessed by con­focal immuno­fluo­res­cen­ce and Western blotting. The histology of the tu­mors was ana­lyzed. IBH-4 and IBH-6 cells were ino­­­cu­lated to nude mice and 100% of the in­jected mi­ce deve­loped tu­mors in the presence or absence of hor­mo­­ne treat­ment, al­though tamo­xifen inhibited growth. IBH-4 and IBH-6 cell lines in vivo gave rise to poorly diffe­­­ren­tiated carci­no­mas with areas of solid growth and sar­co­­ma­toid areas showing no mor­pho­lo­gical signs of epithe­lial differentiation. Distinct fea­tu­res of ma­lignancy were ob­served. IBH-7 tu­mors in animals receiving estradiol were semi-diffe­ren­­tia­ted ade­no­carcinomas. IBH-7 cells grew only in the pre­­sen­ce of estradiol, but even with hormone addition, the tumor take was 20%. The­se tu­mors me­tas­ta­sized to the uterus and lung and vascular tumor emboli we­re evi­dent. IBH-7 tu­mors were invasive and able to break through the pe­ri­­to­neum. As a conclusion, IBH-4 and IBH-6 are good models for studying tu­mor pro­­gres­sion, whereas IBH-7 is a good model for tumor take, being metastatic and stric­tly estro­gen-dependent.
Palabras clave: Breast Cancer , Human Cell Lines , Hormone Receptors
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/24475
DOI: http://dx.doi.org/10.1002/jcp.21694
URL: http://onlinelibrary.wiley.com/doi/10.1002/jcp.21694/abstract
Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Bruzzone, Ariana; Vanzulli, Silvia; Soldati, Rocío Cinthia; Giulianelli, Sebastian Jesus; Lanari, Claudia Lee Malvina; et al.; Novel human breast cancer cell lines IBH-4, IBH-6, and IBH-7 growing in nude mice; Wiley; Journal of Cellular Physiology; 219; 2; 2-2009; 477-484
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