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dc.contributor.author
Piaggio, María Virginia
dc.contributor.author
Peirotti, Marta Beatriz
dc.contributor.author
Deiber, Julio Alcides
dc.date.available
2017-09-15T21:57:34Z
dc.date.issued
2009-06
dc.identifier.citation
Piaggio, María Virginia; Peirotti, Marta Beatriz; Deiber, Julio Alcides; Analysis of the interplay among charge, hydration and shape of proteins through the modeling of their CZE mobility data; Wiley VCH Verlag; Electrophoresis; 30; 13; 6-2009; 2328-2336
dc.identifier.issn
0173-0835
dc.identifier.uri
http://hdl.handle.net/11336/24443
dc.description.abstract
Electrophorectic mobility data of four proteins are analyzed and interpreted through a physicochemical CZE model, which provides estimates of quantities like equivalent hydrodynamic radius (size), effective charge number, shape orientation factor, hydration, actual pK values of ionizing groups, and pH near molecule, among others. Protein friction coefficients are simulated through the creeping flow theory of prolate spheroidal particles. The modeling of the effective electrophoretic mobility of proteins requires consideration of hydrodynamic size and shape coupled to hydration and effective charge. The model proposed predicts native protein hydrations within the range of values obtained experimentally from other techniques. Therefore, this model provides consistently other physicochemical properties such as average friction and diffusion coefficients and packing fractal dimension. As the pH varies from native conditions to those that are denaturing the protein, hydration and packing fractal dimension change substantially. Needs for further research are also discussed and proposed.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Effective Electrical Charge
dc.subject
Hydration
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Hydrodynamic Shape
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Hydrodynamic Size
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Protein Electrophoretic Mobility
dc.subject.classification
Otras Ingeniería Química
dc.subject.classification
Ingeniería Química
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Analysis of the interplay among charge, hydration and shape of proteins through the modeling of their CZE mobility data
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-13T16:09:36Z
dc.journal.volume
30
dc.journal.number
13
dc.journal.pagination
2328-2336
dc.journal.pais
Alemania
dc.description.fil
Fil: Piaggio, María Virginia. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Peirotti, Marta Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Deiber, Julio Alcides. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
Electrophoresis
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/elps.200800743
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.200800743/abstract
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