Artículo
Analysis of the interplay among charge, hydration and shape of proteins through the modeling of their CZE mobility data
Fecha de publicación:
06/2009
Editorial:
Wiley VCH Verlag
Revista:
Electrophoresis
ISSN:
0173-0835
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Electrophorectic mobility data of four proteins are analyzed and interpreted through a physicochemical CZE model, which provides estimates of quantities like equivalent hydrodynamic radius (size), effective charge number, shape orientation factor, hydration, actual pK values of ionizing groups, and pH near molecule, among others. Protein friction coefficients are simulated through the creeping flow theory of prolate spheroidal particles. The modeling of the effective electrophoretic mobility of proteins requires consideration of hydrodynamic size and shape coupled to hydration and effective charge. The model proposed predicts native protein hydrations within the range of values obtained experimentally from other techniques. Therefore, this model provides consistently other physicochemical properties such as average friction and diffusion coefficients and packing fractal dimension. As the pH varies from native conditions to those that are denaturing the protein, hydration and packing fractal dimension change substantially. Needs for further research are also discussed and proposed.
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Articulos(INTEC)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Articulos de INST.DE DES.TECNOL.PARA LA IND.QUIMICA (I)
Citación
Piaggio, María Virginia; Peirotti, Marta Beatriz; Deiber, Julio Alcides; Analysis of the interplay among charge, hydration and shape of proteins through the modeling of their CZE mobility data; Wiley VCH Verlag; Electrophoresis; 30; 13; 6-2009; 2328-2336
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