Mostrar el registro sencillo del ítem
dc.contributor.author
Alvarez, Diego Ezequiel
dc.contributor.author
Agaisse, Herve
dc.date.available
2017-09-15T15:47:05Z
dc.date.issued
2013-09-23
dc.identifier.citation
Alvarez, Diego Ezequiel; Agaisse, Herve; The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility; Rockefeller Univ Press; Journal Of Cell Biology; 202; 7; 23-9-2013; 1075-1090
dc.identifier.issn
0021-9525
dc.identifier.uri
http://hdl.handle.net/11336/24356
dc.description.abstract
Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP– ARP2/3 and Rac1–FHOD1 pathways.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Rockefeller Univ Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Vaccinia Virus
dc.subject
Formin
dc.subject
Actin
dc.subject
Rac1
dc.subject
Fhod1
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-09-14T13:55:11Z
dc.journal.volume
202
dc.journal.number
7
dc.journal.pagination
1075-1090
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Alvarez, Diego Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. University of Yale. School of Medicine; Estados Unidos
dc.description.fil
Fil: Agaisse, Herve. University of Yale. School of Medicine; Estados Unidos
dc.journal.title
Journal Of Cell Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jcb.rupress.org/content/202/7/1075
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1083/jcb.201303055
Archivos asociados