Artículo
The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility
Fecha de publicación:
23/09/2013
Editorial:
Rockefeller Univ Press
Revista:
Journal Of Cell Biology
ISSN:
0021-9525
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Vaccinia virus dissemination relies on the N-WASP– ARP2/3 pathway, which mediates actin tail formation underneath cell-associated extracellular viruses (CEVs). Here, we uncover a previously unappreciated role for the formin FHOD1 and the small GTPase Rac1 in vaccinia actin tail formation. FHOD1 depletion decreased the number of CEVs forming actin tails and impaired the elongation rate of the formed actin tails. Recruitment of FHOD1 to actin tails relied on its GTPase binding domain in addition to its FH2 domain. In agreement with previous studies showing that FHOD1 is activated by the small GTPase Rac1, Rac1 was enriched and activated at the membrane surrounding actin tails. Rac1 depletion or expression of dominant-negative Rac1 phenocopied the effects of FHOD1 depletion and impaired the recruitment of FHOD1 to actin tails. FHOD1 overexpression rescued the actin tail formation defects observed in cells overexpressing dominant-negative Rac1. Altogether, our results indicate that, to display robust actin-based motility, vaccinia virus integrates the activity of the N-WASP– ARP2/3 and Rac1–FHOD1 pathways.
Palabras clave:
Vaccinia Virus
,
Formin
,
Actin
,
Rac1
,
Fhod1
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Identificadores
Colecciones
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Alvarez, Diego Ezequiel; Agaisse, Herve; The formin FHOD1 and the small GTPase Rac1 promote vaccinia virus actin-based motility; Rockefeller Univ Press; Journal Of Cell Biology; 202; 7; 23-9-2013; 1075-1090
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