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dc.date.available
2024-08-27T10:32:37Z
dc.identifier.citation
Saran, Anabel; (2024): Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/243156
dc.identifier.uri
http://hdl.handle.net/11336/243156
dc.description.abstract
In-Vitro Trace Element Uptake by Bacteria in Liquid Cultures-Scanning Electron Microscopy (SEM-EDX) Analysis. Bacillus paramycoides ST4, Bacillus wiedmannii ST29 and Bacillus proteolyticus ST9 images obtained by SEM-EDX are presented in this dataset. Pb was found present in/on the bacterial cell wall of these bacteria cultures and detected by EDX spectra.
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.title
Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates
dc.type
dataset
dc.date.updated
2024-08-26T13:15:11Z
dc.description.fil
Fil: Saran, Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia; Argentina
dc.datacite.PublicationYear
2024
dc.datacite.Creator
Saran, Anabel
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Confluencia
dc.datacite.affiliation
Consejo Nacional de Investigaciones Científicas y Técnicas
dc.datacite.publisher
Consejo Nacional de Investigaciones Científicas y Técnicas
dc.datacite.subject
Otras Ciencias de la Tierra y relacionadas con el Medio Ambiente
dc.datacite.subject
Ciencias de la Tierra y relacionadas con el Medio Ambiente
dc.datacite.subject
CIENCIAS NATURALES Y EXACTAS
dc.datacite.ContributorType
RelatedPerson
dc.datacite.ContributorName
Merini, Luciano Jose
dc.datacite.date
03/05/2024-22/08/2024
dc.datacite.DateType
Creado
dc.datacite.language
eng
dc.datacite.AlternateIdentifierType
info:eu-repo/semantics/altIdentifier/doi/10.5281/zenodo.11106952
dc.datacite.version
1.0
dc.datacite.description
Batch studies were conducted using 50 mL Falcon tubes that contained 25 mL GY rich medium supplemented with 100 mg L-1 of Pb (Pb(NO3)2). All tubes were inoculated with 1 mL of a bacterial cell suspension (optical density at 600 nm = 1), which was previously grown in GY medium at 28 °C for 24 h. Samples were incubated at 28 °C and 100 rpm on an orbital shaker. In order to estimate Pb sequestration by bacterial cells, cultures were first centrifuged 15 min at 4800 rpm, bacterial pellets were washed 3 times with 0.01 M phosphate-buffer (PBS, pH 7.0) to remove unbound metals, sugars and proteins. The samples were dehydrated with ethanol/water mixtures, until absolute ethanol. Then 100% acetone was used. Pellets were resuspended in 2% glutaraldehyde for 1h at room temperature. Afterwards, bacterial samples were centrifuged for 3 min at 3000 rpm and pellets were washed 3 times with milli-Q water. One uL of sample was placed on a sample holder, in carbon conductive tape. Then, samples were coated 30 s with a 15 nm gold layer and analysed using a Scanning Electron Microscope (Phenom™ ProX Desktop, Thermofisher). Images were taken using an accelerating voltage of 15 kV.
dc.datacite.DescriptionType
Métodos
dc.datacite.FundingReference
RG-16110-ATN/RF
dc.datacite.FunderName
Banco Interamericano de Desarrollo.
dc.relationtype.isSourceOf
https://ri.conicet.gov.ar/handle/11336/168586
dc.relationtype.isSourceOf
https://ri.conicet.gov.ar/handle/11336/174952
dc.subject.keyword
Bioaugmentation
dc.subject.keyword
Plant growth promoting bacteria
dc.subject.keyword
Trace elements
dc.subject.keyword
Polluted soil
dc.datacite.resourceTypeGeneral
dataset
dc.conicet.datoinvestigacionid
19805
dc.datacite.awardTitle
FONTAGRO: Desarrollo de microeconomías regionales en la producción de aceites esenciales cosechadas en suelos mineros
dc.datacite.geolocation
Santa Rosa
dc.datacite.formatedDate
2024
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