Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates

Name: Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates
License: https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Keywords: Bioaugmentation

Datos de investigación

Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates

Autores: Saran, Anabel Icon

Colaboradores: Merini, Luciano Jose Icon

Publicador: Consejo Nacional de Investigaciones Científicas y Técnicas

Fecha de depósito: 27/08/2024

Fecha de creación: 03/05/2024-22/08/2024

Clasificación temática:

Otras Ciencias de la Tierra y relacionadas con el Medio Ambiente

Resumen

In-Vitro Trace Element Uptake by Bacteria in Liquid Cultures-Scanning Electron Microscopy (SEM-EDX) Analysis. Bacillus paramycoides ST4, Bacillus wiedmannii ST29 and Bacillus proteolyticus ST9 images obtained by SEM-EDX are presented in this dataset. Pb was found present in/on the bacterial cell wall of these bacteria cultures and detected by EDX spectra.

Métodos

Batch studies were conducted using 50 mL Falcon tubes that contained 25 mL GY rich medium supplemented with 100 mg L-1 of Pb (Pb(NO3)2). All tubes were inoculated with 1 mL of a bacterial cell suspension (optical density at 600 nm = 1), which was previously grown in GY medium at 28 °C for 24 h. Samples were incubated at 28 °C and 100 rpm on an orbital shaker. In order to estimate Pb sequestration by bacterial cells, cultures were first centrifuged 15 min at 4800 rpm, bacterial pellets were washed 3 times with 0.01 M phosphate-buffer (PBS, pH 7.0) to remove unbound metals, sugars and proteins. The samples were dehydrated with ethanol/water mixtures, until absolute ethanol. Then 100% acetone was used. Pellets were resuspended in 2% glutaraldehyde for 1h at room temperature. Afterwards, bacterial samples were centrifuged for 3 min at 3000 rpm and pellets were washed 3 times with milli-Q water. One uL of sample was placed on a sample holder, in carbon conductive tape. Then, samples were coated 30 s with a 15 nm gold layer and analysed using a Scanning Electron Microscope (Phenom™ ProX Desktop, Thermofisher). Images were taken using an accelerating voltage of 15 kV.

Palabras clave: Bioaugmentation, Plant growth promoting bacteria, Trace elements, Polluted soil

Alcance geográfico

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Identificador del recurso

URI: http://hdl.handle.net/11336/243156

Colecciones

Datos de Investigación(CCT - PATAGONIA CONFLUENCIA)
Datos de Investigación de CTRO.CIENTIFICO TECNOL.CONICET - PATAGONIA CONFLUENCIA

Citación

Saran, Anabel; (2024): Scanning electron microscopy (SEM) images of single bacterial-Pb tolerant isolates. Consejo Nacional de Investigaciones Científicas y Técnicas. (dataset). http://hdl.handle.net/11336/243156

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)