Evento
Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin
Alesso, Magdalena
; Santarossa, Debora Gimena
; Kaplan, Marcos Manuel
; Talio, María Carolina
; Acosta, Maria Gimena
; Fernandez, Liliana Patricia
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Tipo del evento:
Congreso
Nombre del evento:
3ra Reunión Internacional de Ciencias Farmacéuticas
Fecha del evento:
18/09/2014
Institución Organizadora:
Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica;
Título de la revista:
International Journal of Pharmaceutical Sciences and Research
Editorial:
Universidad Nacional de Córdoba
ISSN:
0975-8232
Idioma:
Inglés
Clasificación temática:
Resumen
Caffeine (1,3,7-trimethylxanthine; CF), is a substance found naturally in the leaves, beans and fruits of a variety of plants. Although it is regularly consumed by a large percentage of adults, in Argentina recently the young have been added to this group. Attending to the health risks, many countries have started action to establish the regulatory boundaries around CF. A new method for the determination of caffeine based on the quenching of Bovine serum albumin (BSA) fluorescence. When the alkaloid CF is presents (quencher), the protein?s fluorescence emission is diminished. The diminution of the signal is proportional at caffeine concentration. The effect of experimental parameters, including the nature of the buffer and pH, and the nature of the surfactant and concentration were investigated by means univariation assays. Under optimum experimental conditions, a detection and quantification limits of 1.9 and 6.4 mg l−1 were obtained, respectively. A linear range was achieved varying from concentrations of 6.4 to 94 mg l−1 (r2 = 0.997). Satisfactory recovery values were obtained using the method of standard addition, confirming the feasibility of this method for caffeine determination in energising dietary supplements and energy drinks. In order to obtain the known advantages of on-line procedure, a flow injection manifold will be sketched and quality analytical parameter will be evaluated.
Palabras clave:
CAFFEINE MONITORING
,
BOVINE SERUM ALBUMIN
,
FLUORESCENT QUENCHING
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Eventos(INQUISAL)
Eventos de INST. DE QUIMICA DE SAN LUIS
Eventos de INST. DE QUIMICA DE SAN LUIS
Citación
Caffeine quantification in dietary supplements by fluorescence using bovine serum albumin; 3ra Reunión Internacional de Ciencias Farmacéuticas; Córdoba; Argentina; 2014; 52-52
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