Artículo
Deoxynivalenol and nivalenol analysis in soybean and soy flour
Barros, Germán Gustavo
; García, Daiana
; Oviedo, S.; Ramirez, Maria Laura
; Torres, Adriana Mabel
; Chulze, Sofia Noemi
Fecha de publicación:
08/2008
Editorial:
Wageningen Academic Publishers
Revista:
World Mycotoxin Journal
ISSN:
1875-0710
e-ISSN:
1875-0796
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
A rapid and accurate method of quantifying deoxynivalenol (DON) and nivalenol (NIV) in soybean and soy flour is described. The samples were extracted with acetonitrile:water (84:16, v/v) and cleaned through a solid phase extraction (SPE) column. The mycotoxins were separated, detected and quantified by reversed-phase high performance liquid chromatography (HPLC) with UV detection (220 nm) using water:methanol (88:12, v/v) as mobile phase. Characteristics of this in-house method such as accuracy, precision and detection and quantification limits were defined by means of a recovery test with spiked soybean and soy flour samples. The detection limit (LOD) was 0.1 μg/g for DON and 0.2 μg/g for NIV, based on a signal-noise ratio 3:1. Quantification limit (LOQ) was established as three times the detection limit.
Palabras clave:
Soybean
,
Trichothecenes
,
Deoxynivalenol
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Barros, Germán Gustavo; García, Daiana; Oviedo, S.; Ramirez, Maria Laura; Torres, Adriana Mabel; et al.; Deoxynivalenol and nivalenol analysis in soybean and soy flour; Wageningen Academic Publishers; World Mycotoxin Journal; 1; 3; 8-2008; 263-266
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