Artículo
Purification and Partial Characterization of Novel Bacteriocin L23 Produced by Lactobacillus fermentum L23
Pascual, Liliana Myriam; Daniele, María B.; Giordano, Walter Fabian
; Pájaro, María C.; Barberis, Isabel Lucila
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
12/2008
Editorial:
Springer
Revista:
Current Microbiology
ISSN:
0343-8651
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Lactobacillus fermentum strain L23 produced a small bacteriocin, designated bacteriocin L23, with an estimated molecular mass of <7,000 Da. Isolation, purification, and partial characterization of bacteriocin L23 are described. It displayed a wide inhibitory spectrum including both Gram-negative and Gram-positive pathogenic strains, and two species of Candida. The antibacterial activity of cell-free culture supernatant fluid was not affected by catalase or urease, but was abolished by the proteolytic enzymes trypsin and protease VI. Bacteriocin L23 was heat-stable (60 min at 100ºC) and showed inhibitory activity over a wide pH range (4.0 to 7.0). The proteinaceous compound was isolated from cell-free culture supernatant fluid and purified. Crude bacteriocin sample was prepared by a process of ammonium sulfate precipitation, gel filtration, thin-layer chromatography (TLC), bioautography, and reversed-phase HPLC.
Palabras clave:
LACTOBACILLUS
,
BACTERIOCIN
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Articulos(CCT - CORDOBA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CORDOBA
Citación
Pascual, Liliana Myriam; Daniele, María B.; Giordano, Walter Fabian; Pájaro, María C.; Barberis, Isabel Lucila; Purification and Partial Characterization of Novel Bacteriocin L23 Produced by Lactobacillus fermentum L23; Springer; Current Microbiology; 56; 4; 12-2008; 397-402
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