Evento
Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions
Gitman, Iván Federico Berco
; Martinez Moyano, Edgar
; Grossi, Cecilia Eugenia María
; Ulloa, Rita Maria
Tipo del evento:
Reunión
Nombre del evento:
LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research
Fecha del evento:
08/11/2022
Institución Organizadora:
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular;
Título de la revista:
Biocell
Editorial:
Tech Science Press
ISSN:
0327-9545
e-ISSN:
1667-5746
Idioma:
Inglés
Clasificación temática:
Resumen
Analysis of the potato genome (SPUD DB) allowed us to identify 142 protease inhibitors (PIs); 42 PI genes are clustered in tandem in chr.3, position 43 Mbp (3.43): 14 encode Kunitz-type PIs (KTIs), 4 encode trypsin type PIs (TPI) and 1 encodes a cystein PI (CPI), and at position 48 MBp (3.48), 10 genes encode KTIs, 8 encode PIN-II and 5 encode serine type PI (PIN-I). On the other hand, 11 genes encoding 5 KTIs, 3 cystatin B, and 3 PIN-I are grouped in Chr. 6, and 12 PIN-I genes are in chr. 9. This multigene family regulates protein turn-over preventing catabolism of essential proteins during metabolic processes and plays a role in the defense against heterologous proteases from pathogens and pests. PIs have been proposed as an alternative to chemical pesticides for the control of herbivorous insects, and as abiotic stress-protective factors. Degenerate primers were designed against five PIs (Soltu.DM.03G018480, -G018520, -G018580, - G018620, -G018650) of 3.43, seven PIs (Soltu.DM.03G023490, -G023500, -G23510, -G23520, -G23530, - G23540, and -G024110) of 3.48, and seven PIs of Chr. 9 (Soltu.DM.09G025840, -G025850 -G025860 - G025880, -G025900, -G025910, and -G025930) that share at least 83.46, 55,2 and 77,53 % of nucleotide identity. According to RNAseq data these three gene clusters are induced upon drought, salt, mannitol and N2 fertilization, and are downregulated during tuber dormancy release. To confirm the RNAseq data, RT-qPCR assays were performed using RNAs from in-vitro plants cultured under control conditions or a) with different NH4+ and NO3- concentrations, b) exposed during 1 week to continuous darkness, and c) to 150 mM NaCl during 24 and 96 h. In addition, RNA was extracted from greenhouse plants exposed to drought conditions. To analyze, the role of PIs during source-sink processes, RNA was obtained from dormant and sprouting tubers, from etiolated and green sprouts, and from young, fully developed and senescent leaves. In the future we aim to characterize one gene of each cluster in overexpressing or knockdown plants to elucidate their potential as biotechnological tools.
Palabras clave:
PROTEASE INHIBITORS
,
POTATO
,
STRESS RESPONSE
,
BIOTECHNOLOGY
Archivos asociados
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Colecciones
Eventos(INGEBI)
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Eventos de INST.DE INVEST.EN ING.GENETICA Y BIOL.MOLECULAR "DR. HECTOR N TORRES"
Citación
Expression analysis of Protease inhibitors in potato plants exposed to different environmental conditions; LVIII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; Mendoza; Argentina; 2022; 1-1
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