Bacterial diets are able to modulate life-history treats in C. elegans models of neurodegenerative diseases

Abstract

As life expectancy increase, age-related disorders, such us neurodegenerative diseases (ND), have become more prevalent. Moreover, treatments only attenuate some symptoms, but fail to arrest characteristic neuronal proteotoxicity. Thus, new challenges emerge to science in order to understand molecular basis of these disorders. Lately, the gut-brain axis has gain attention and a close relation between gut microorganism and ND has been proposed. The aim of our work was to evaluate the relevance of the microbiota in the progression of proteotoxic-based disorders, assessing the impact of six non-pathogenic bacterial diets on life-history traits in C. elegans models of ND (vs standard OP50). In a first approach, we found 2 bacteria, Escherichia coli K12 and E. coli HB101, able to improve locomotion in liquid media, in worm’s model of Parkinson disease (PD) at adult day 4, versus E. coli OP50. Moreover, an age-dependent locomotion improvement, between larva-L4 and adult day 4, was observed in solid media after feeding PD model´s worms with 4 different bacteria versus E. coli OP50. We also observed an increase in the developmental timing of wild-type worms grown in 4 bacteria versus E. coli OP50, but more interesting was the accelerated developmental rate selectively found in models of PD and Huntington disease feed with E. coli BL21 (DE3). We are currently evaluating aggregate numbers, lifespan and mitochondrial morphology among others. Our results allowed us to identify bacteria with the ability to drive physiological outcomes and improve health status of C. elegans models of neurodegenerative diseases.