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dc.contributor.author Mammana, Sabrina Belén
dc.contributor.author Berton, Paula
dc.contributor.author Camargo, Alejandra Beatriz
dc.contributor.author Lascalea, Gustavo Enrique
dc.contributor.author Altamirano, Jorgelina Cecilia
dc.date.available 2017-09-07T17:04:48Z
dc.date.issued 2017-03-20
dc.identifier.citation Mammana, Sabrina Belén; Berton, Paula; Camargo, Alejandra Beatriz; Lascalea, Gustavo Enrique; Altamirano, Jorgelina Cecilia; Coprecipitation-assisted coacervative extraction coupled to high-performance liquid chromatography: An approach for determining organophosphorus pesticides in water samples; Wiley VCH Verlag; Electrophoresis; 38; 9-10; 20-3-2017; 1334-1343
dc.identifier.issn 0173-0835
dc.identifier.uri http://hdl.handle.net/11336/23770
dc.description.abstract An analytical methodology based on coprecipitation assisted coacervative extraction cop-CAE) coupled to High Performance Liquid Chromatography and UV-visible detection was developed for analysis of five organophosphorus pesticides (OPPs), including fenitrothion, guthion, parathion, methidathion and chlorpyrifos, in water samples. It is the first time that cop-CAE was used for extraction and preconcentration of OPPs from water for further determination by HPLC. The effects of relevant physic-chemical variables on the analytical response of each OPP (pH, precipitant concentration, surfactant concentration, stirring time, equilibration time and temperature) were studied and optimized, as well as the working conditions for coupling cop-CAE to HPLC-UV. Under optimized conditions, the resulting methodology was as follows: an aliquot of 10 mL of water sample was placed into a 15 mL centrifuge tube where 0.5 mL of sodium citrate 0.1 M, pH 4, 0.08 mL of aluminum sulfate 0.1 mM and 0.7 mL of SDS 0.1 mM were added and homogenized for 8 min using a vortex stirrer to induce the formation of the admicelles. The resulting cloudy solution was kept at 25 °C for 5 min for equilibration. After centrifugation the coacervate rich-phase and supernatant were separated. The resulting supernatant was removed and discarded. A 0.7 mL aliquot of the coacervate rich-phase was transferred to 2 mL vial and dissolved with 300 µL of methanol. An aliquot of 20 µL of the resulting homogeneous solution was injected into the HPLC-UV for analysis. The method detection limits for fenitrothion, parathion, guthion, methidathion and chlorpyrifos under optimized conditions was 83, 35, 35, 57 and 12 ng L-1, respectively. The RSD (%) for the studied analytes were < 3% (n=3). Recovery values ranged from 78 to 93%. The proposed analytical methodology was successfully applied for the analysis of five OPPs in different water sample of Mendoza city.
dc.format application/pdf
dc.language.iso eng
dc.publisher Wiley VCH Verlag
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject organophosphorus pesticides
dc.subject cop-CAE
dc.subject water samples
dc.subject HPLC-UV.
dc.subject Coprecipitation-assisted coacervative extraction / Green chemistry / HPLC / Organophosphorus pesticides / Water samples
dc.subject coacervative extraction
dc.subject Green chemistry
dc.subject HPLC
dc.subject.classification Otras Ciencias Químicas
dc.subject.classification Ciencias Químicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Coprecipitation-assisted coacervative extraction coupled to high-performance liquid chromatography: An approach for determining organophosphorus pesticides in water samples
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T17:54:21Z
dc.journal.volume 38
dc.journal.number 9-10
dc.journal.pagination 1334-1343
dc.journal.pais Alemania
dc.journal.ciudad Weinheim
dc.description.fil Fil: Mammana, Sabrina Belén. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil Fil: Berton, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
dc.description.fil Fil: Camargo, Alejandra Beatriz. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Laboratorio de Cromatografía para Agroalimentos ; Argentina
dc.description.fil Fil: Lascalea, Gustavo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina
dc.description.fil Fil: Altamirano, Jorgelina Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Provincia de Mendoza. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales. Universidad Nacional de Cuyo. Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales; Argentina. Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales; Argentina
dc.journal.title Electrophoresis
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/elps.201600335/abstract
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/elps.201600335


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)