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Artículo

Coprecipitation-assisted coacervative extraction coupled to high-performance liquid chromatography: An approach for determining organophosphorus pesticides in water samples

Mammana, Sabrina Belén; Berton, PaulaIcon ; Camargo, Alejandra BeatrizIcon ; Lascalea, Gustavo EnriqueIcon ; Altamirano, Jorgelina CeciliaIcon
Fecha de publicación: 20/03/2017
Editorial: Wiley VCH Verlag
Revista: Electrophoresis
ISSN: 0173-0835
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
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Resumen

An analytical methodology based on coprecipitation assisted coacervative extraction cop-CAE) coupled to High Performance Liquid Chromatography and UV-visible detection was developed for analysis of five organophosphorus pesticides (OPPs), including fenitrothion, guthion, parathion, methidathion and chlorpyrifos, in water samples. It is the first time that cop-CAE was used for extraction and preconcentration of OPPs from water for further determination by HPLC. The effects of relevant physic-chemical variables on the analytical response of each OPP (pH, precipitant concentration, surfactant concentration, stirring time, equilibration time and temperature) were studied and optimized, as well as the working conditions for coupling cop-CAE to HPLC-UV. Under optimized conditions, the resulting methodology was as follows: an aliquot of 10 mL of water sample was placed into a 15 mL centrifuge tube where 0.5 mL of sodium citrate 0.1 M, pH 4, 0.08 mL of aluminum sulfate 0.1 mM and 0.7 mL of SDS 0.1 mM were added and homogenized for 8 min using a vortex stirrer to induce the formation of the admicelles. The resulting cloudy solution was kept at 25 °C for 5 min for equilibration. After centrifugation the coacervate rich-phase and supernatant were separated. The resulting supernatant was removed and discarded. A 0.7 mL aliquot of the coacervate rich-phase was transferred to 2 mL vial and dissolved with 300 µL of methanol. An aliquot of 20 µL of the resulting homogeneous solution was injected into the HPLC-UV for analysis. The method detection limits for fenitrothion, parathion, guthion, methidathion and chlorpyrifos under optimized conditions was 83, 35, 35, 57 and 12 ng L-1, respectively. The RSD (%) for the studied analytes were < 3% (n=3). Recovery values ranged from 78 to 93%. The proposed analytical methodology was successfully applied for the analysis of five OPPs in different water sample of Mendoza city.
Palabras clave: Organophosphorus Pesticides , Cop-Cae , Water Samples , Hplc-Uv. , Coprecipitation-Assisted Coacervative Extraction / Green Chemistry / Hplc / Organophosphorus Pesticides / Water Samples , Coacervative Extraction , Green Chemistry , Hplc
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/23770
URL: http://onlinelibrary.wiley.com/doi/10.1002/elps.201600335/abstract
DOI: http://dx.doi.org/10.1002/elps.201600335
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Articulos(IBAM)
Articulos de INST.DE BIOLOGIA AGRICOLA DE MENDOZA
Citación
Mammana, Sabrina Belén; Berton, Paula; Camargo, Alejandra Beatriz; Lascalea, Gustavo Enrique; Altamirano, Jorgelina Cecilia; Coprecipitation-assisted coacervative extraction coupled to high-performance liquid chromatography: An approach for determining organophosphorus pesticides in water samples; Wiley VCH Verlag; Electrophoresis; 38; 9-10; 20-3-2017; 1334-1343
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