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Artículo

Glial Cell-Elicited Activation of Brain Microvasculature in Response to Brucella abortus Infection Requires ASC Inflammasome-Dependent IL-1β Production

Miraglia, Maria CruzIcon ; Franco, Miriam M. Costa; Rodríguez, Ana MaríaIcon ; Bellozi, Paula M. Q.; Ferrari, Carina CintiaIcon ; Farias, Maria IsabelIcon ; Dennis, Vida A.; Barrionuevo, PaulaIcon ; Oliveira, Antonio C. P. de; Pitossi, Fernando JuanIcon ; Kim, Kwang Sik; Delpino, María VictoriaIcon ; Oliveira, Sergio C.; Giambartolomei, Guillermo HernanIcon
Fecha de publicación: 03/2016
Editorial: American Association of Immunologists
Revista: Journal of Immunology
ISSN: 0022-1767
e-ISSN: 1550-6606
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioquímica y Biología Molecular

Resumen

Blood-brain barrier activation and/or dysfunction are a common feature of human neurobrucellosis, but the underlying pathogenic mechanisms are largely unknown. In this article, we describe an immune mechanism for inflammatory activation of human brain microvascular endothelial cells (HBMEC) in response to infection with Brucella abortus Infection of HBMEC with B. abortus induced the secretion of IL-6, IL-8, and MCP-1, and the upregulation of CD54 (ICAM-1), consistent with a state of activation. Culture supernatants (CS) from glial cells (astrocytes and microglia) infected with B. abortus also induced activation of HBMEC, but to a greater extent. Although B. abortus-infected glial cells secreted IL-1β and TNF-α, activation of HBMEC was dependent on IL-1β because CS from B. abortus-infected astrocytes and microglia deficient in caspase-1 and apoptosis-associated speck-like protein containing a CARD failed to induce HBMEC activation. Consistently, treatment of CS with neutralizing anti-IL-1β inhibited HBMEC activation. Both absent in melanoma 2 and Nod-like receptor containing a pyrin domain 3 are partially required for caspase-1 activation and IL-1β secretion, suggesting that multiple apoptosis-associated speck-like protein containing CARD-dependent inflammasomes contribute to IL-1β-induced activation of the brain microvasculature. Inflammasome-mediated IL-1β secretion in glial cells depends on TLR2 and MyD88 adapter-like/TIRAP. Finally, neutrophil and monocyte migration across HBMEC monolayers was increased by CS from Brucella-infected glial cells in an IL-1β-dependent fashion, and the infiltration of neutrophils into the brain parenchyma upon intracranial injection of B. abortus was diminished in the absence of Nod-like receptor containing a pyrin domain 3 and absent in melanoma 2. Our results indicate that innate immunity of the CNS set in motion by B. abortus contributes to the activation of the blood-brain barrier in neurobrucellosis and IL-1β mediates this phenomenon.
Palabras clave: Hbmec , Brucella , Neurobrucellosis , Inflammasome
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/23627
URL: http://www.jimmunol.org/content/196/9/3794
DOI: https://doi.org/10.4049/jimmunol.1500908
Colecciones
Articulos(IIBBA)
Articulos de INST.DE INVEST.BIOQUIMICAS DE BS.AS(I)
Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Miraglia, Maria Cruz; Franco, Miriam M. Costa; Rodríguez, Ana María; Bellozi, Paula M. Q.; Ferrari, Carina Cintia; et al.; Glial Cell-Elicited Activation of Brain Microvasculature in Response to Brucella abortus Infection Requires ASC Inflammasome-Dependent IL-1β Production; American Association of Immunologists; Journal of Immunology; 196; 9; 3-2016; 3794-3805
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