Single and joint toxicity of azoxystrobin and cyproconazole to Prochilodus lineatus: Bioconcentration and biochemical responses

Abstract

Fungicides are widely used across the world to protect crops and their presence in freshwater systems is increasing. However, the evaluation of their potential impacts on non-target organisms is in the minority of studies related to pesticides. In the current research, the single and joint toxicity of azoxystrobin (AZX) and cyproconazole (CYP) was investigated in juvenile fish Prochilodus lineatus. In particular, we evaluated bioconcentration and biochemical responses following a short-term exposure to environmentally relevant concentrations of the fungicides (alone and in mixture). We also determined interactions between the biological responses when the two compounds were used in mixture. Our results demonstrate that AZX and CYP pose a risk to native freshwater fish by causing deleterious effects. Both compounds, alone and in mixture, bioaccumulated in P. lineatus and triggered neurotoxicity and changes in oxidative stress biomarkers in several organs. Moreover, muscle was a target tissue for these fungicides and a synergistic interaction was observed for the mixture. Due to the lack of studies in fish assessing the effects following exposure to AZX-CYP mixtures and considering a realistic exposure situation in agriculture-impacted water bodies, these findings provide new and relevant information for future studies.