Artículo
Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom
Leiva, Carlos Leónidas
; Cangelosi, Adriana; Mariconda, Virginia Laura; Celi, Ana; Joaquim, Patricia Estefania; Geoghegan, Patricia; Fernandez Miyakawa, Mariano Enrique
; Chacana, Pablo Anibal
Fecha de publicación:
02/2023
Editorial:
Springer
Revista:
Applied Microbiology and Biotechnology
ISSN:
0175-7598
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The use of egg yolk antibodies—IgY technology—represents an alternative to the production of mammalian immunoglobulins and has several advantages regarding animal welfare and lower costs of production. The use of adjuvants to achieve the hyperimmunization of laying hens plays a key role in the success of the production of high levels of the antibodies. In the present work, two different adjuvant systems (Freund’s adjuvants and MontanideTM ISA 71 VG) were compared to produce IgY anti-Bothrops alternatus. For the first immunization, formalin-inactivated Salmonella was added to MontanideTM ISA 71 VG to emulate Freund’s complete adjuvant which includes a mycobacteria antigen. After eight immunizations, IgY produced by using either adjuvant was able to neutralize the lethal activity of the venom in a mouse model, but differences were found regarding the recognition of components of the venom between the two adjuvants tested. Overall, MontanideTM adjuvant used in this work could be a good alternative choice to produce antibodies capable of neutralizing the lethality of complex antigens. This adjuvant is commercially available and used in the formulation of several poultry vaccines and could be used for the IgY technology instead of traditional immunomodulators such as Freund’s adjuvants. Key points • IgY extracts recognized major components of the venom. • Avidity indexes of the IgY extracts increased after the successive immunizations. • IgY obtained by two adjuvant systems neutralized the lethal activity of the venom.
Palabras clave:
ADJUVANT
,
EGG YOLK
,
IGY ANTIBODY
,
MASS SPECTROMETRY
,
MONTANIDE
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IPVET)
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Citación
Leiva, Carlos Leónidas; Cangelosi, Adriana; Mariconda, Virginia Laura; Celi, Ana; Joaquim, Patricia Estefania; et al.; Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom; Springer; Applied Microbiology and Biotechnology; 107; 5-6; 2-2023; 1947-1957
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