Production of bacterial nanocellulose in alternative culture media under static and dynamic conditions

Abstract

Bacterial nanocellulose (BNC) was produced in three different culture media: the stand-ard HS medium, analytical glycerol/corn steep liquor (AG-CSL) medium and glycerol remaining from bio-diesel production/corn steep liquor (BG-CSL) me-dium. Both static and agitated conditions were as-sessed, and the effects of the culture medium, agita-tion speed and cultivation time used were evaluated on BNC production and selected products character-istics (morphology, specific surface area and crystal-linity). Results showed that the cheaper alternative culture media assayed led to increased production of BNC, and that agitation speed modulation allowed producing either BNC pellicles or BNC pellets with varying size, shape, crystallinity and cellulose nano-fibers packing.