Artículo
His-tagged lactate oxidase production for industrial applications using fed-batch fermentation
Godino, Agustina
; Amaranto, Marilla
; Manassero, Agustina
; Comba, Fausto Nahuel
; Alonzo Pérez, Martín; Simonella, Lucio Esteban
; Martín Pernigotti; Barra, Jose Luis
Fecha de publicación:
02/2023
Editorial:
Elsevier Science
Revista:
Journal of Biotechnology
ISSN:
0168-1656
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
L-lactate oxidase (LOX) is a biotechnologically important enzyme used in biosensors and colorimetric kits to detect lactate, a key biomarker in clinical diagnostics, sports medicine and the food industry. In this work, we produced a recombinant His-tagged Aerococcus viridans LOX (rLOX) in Escherichia coli and carried out its functional characterization for industrial applications. Our rLOX was evaluated in a colorimetric kit for human diagnostics and in an amperometric biosensor to measure the lactic acid in food products. The rLOX was fully functional for both applications, with a performance comparable to commercial untagged LOXs. As the industrial use of LOX enzyme requires a large-scale production, we scaled up the rLOX production in a fed-batch bioreactor culture and obtained a yield approximately ten times higher than that of the Erlenmeyer scale. The His-tag allowed an easy and highly efficient purification process, and a high-purity rLOX was recovered after this one-step affinity purification. In this study, we described a simple, rapid and cost-competitive approach for the production of a recombinant His-tagged LOX enzyme suitable for industrial use.
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Articulos (IMITAB)
Articulos de INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIÓN Y TRANSFERENCIA AGROALIMENTARIA Y BIOTECNOLÓGICA
Articulos de INSTITUTO MULTIDISCIPLINARIO DE INVESTIGACIÓN Y TRANSFERENCIA AGROALIMENTARIA Y BIOTECNOLÓGICA
Articulos(CIQUIBIC)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Articulos de CENTRO DE INVEST.EN QCA.BIOL.DE CORDOBA (P)
Citación
Godino, Agustina; Amaranto, Marilla; Manassero, Agustina; Comba, Fausto Nahuel; Alonzo Pérez, Martín; et al.; His-tagged lactate oxidase production for industrial applications using fed-batch fermentation; Elsevier Science; Journal of Biotechnology; 363; 2-2023; 1-7
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