Artículo
Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
Colombatti Olivieri, María Alejandra
; Fresia, P.; Graña, M.; Cuerda, Maria Ximena
; Nagel, Ariel Gastón
; Alvarado Pinedo, María Fiorella; Romano, Maria Isabel
; Caimi, Karina Cynthia
; Berná, L.; Santangelo, María de la Paz
Fecha de publicación:
12/2022
Editorial:
Churchill Livingstone
Revista:
Tuberculosis (Edinb)
ISSN:
1472-9792
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.
Palabras clave:
GENOMICS
,
OXIDATIVE STRESS
,
PHENOTYPE
,
VIRULENCE
,
WGS
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IABIMO)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA Y BIOLOGIA MOLECULAR
Citación
Colombatti Olivieri, María Alejandra; Fresia, P.; Graña, M.; Cuerda, Maria Ximena; Nagel, Ariel Gastón; et al.; Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype; Churchill Livingstone; Tuberculosis (Edinb); 138; 12-2022; 1-45
Compartir
Altmétricas
Items relacionados
Mostrando titulos relacionados por título, autor y tema.
-
Torres, Carolina ; Mojsiejczuk, Laura Noelia ; Acuña, Dolores ; Alexay, Sofía; Amadio, Ariel Fernando ; Aulicino, Paula ; Debat, Humberto Julio ; Fay, Fabian; Fernández, Franco; Giri, Adriana Angelica ; Goya, Stephanie ; König, Guido Alberto ; Lucero, Horacio; Nabaes Jodar, Mercedes Soledad ; Pianciola, Luis; Sfalcin, Javier A.; Acevedo, Raúl Maximiliano ; Bengoa Luoni, Sofia Ailin ; Bolatti, Elisa Maria ; Brusés, Bettina Laura; Cacciabue, Marco Polo Domingo ; Casal, Pablo Ezequiel; Cerri, Agustina ; Chouhy, Diego ; Dus Santos, María José; Eberhardt, Maria Florencia ; Fernández, Ailén; Fernández, Paula del Carmen ; Fernández Do Porto, Darío Augusto ; Formichelli, Laura Belén; Gismondi, María Inés; Irazoqui, José Matías ; Lorenzini Campos, Melina Noelia ; Lusso, Silvina; Marquez, Nathalie ; Muñoz, Marianne; Mussin, Javier Esteban ; Natale, Mónica Inés; Oria, Griselda Ines; Pisano, María Belén ; Posner, Victoria Maria; Puebla, Andrea; Ré, Viviana Elizabeth ; Sosa, Ezequiel ; Villanova, Gabriela Vanina ; Zaiat, Jonathan Javier ; Zunino, Sebastián; Acevedo, María Elina; Acosta, Julián; Alvarez Lopez, Cristina; Álvarez, María Laura; Angeleri, Patricia; Angelletti, Andrés; Arca, Manuel; Ayala, Natalia A.; Barbas, Maria Gabriela; Bertone, Ana; Bonnet, Maria Agustina; Bourlot, Ignacio; Cabassi, María Victoria; Castello, Alejandro; Castro, Gonzalo; Cavatorta, Ana Laura ; Ceriani, Maria Carolina ; Cimmino, Carlos José; Cipelli, Julián; Colmeiro, María; Cordero, Andrés; Cristina, Silvia Carolina ; Di Bella, Sofia; Dolcini, Guillermina Laura ; Ercole, Regina; Espasandin, Yesica Romina ; Espul, Carlos; Falaschi, Andrea; Fernández Moll, Facundo Lucio; Foussal, María Delia; Gatelli, Andrea; Goñi, Sandra Elizabeth ; Jofré, María Estela; Jaramillo Ortiz, José Manuel ; Labarta, Natalia; Lacaze, María Agustina; Larreche Calahorrano, María Rocío; Leiva, Viviana; Levin, Gustavo Javier ; Luczak, Erica Natalia; Mandile, Marcelo Gastón ; Marino, Gioia; Massone, Carla Antonella; Mazzeo, Melina; Medina, Carla; Monaco, Belén; Montoto, Luciana; Mugna, Viviana; Musto, Alejandra Beatriz; Nadalich, Victoria; Nieto Farías, María Victoria ; Ojeda, Guillermo; Piedrabuena, Andrea C.; Pintos, Carolina; Pozzati, Marcia; Rahhal, Marilina; Rechimont, Claudia; Remes Lenicov, Federico ; Rompato, Gabriela; Seery, Vanesa ; Siri, Leticia; Spina, Maria Julieta; Streitenberger, Cintia; Suárez, Ariel; Suárez, Jorgelina; Sujansky, Paula; Talia, Juan Manuel ; Theaux, Clara; Thomas, Guillermo; Ticeira, Marina; Tittarelli, Estefanía ; Toro, Rosana; Uez, Osvaldo; Zaffanella, María Belén; Ziehm, Cecilia; Zubieta, Martin; Mistchenko, Alicia Susana; Valinotto, Laura Elena ; Viegas, Mariana (Frontiers Media, 2021-12)
-
Alfonso, María Belén; Scordo, Facundo ; Epele, Luis Beltran ; Isobe, Atsuhiko; Akmentins, Mauricio Sebastián ; Albariño, Ricardo Javier ; Albarrán, Katya Karla; Allende, Luz ; Andrade Muñoz, Alan Sebastian ; Ansoar Rodríguez, Yadira ; Arias, Andres Hugo ; Balseiro, Esteban Gabriel ; Berenstein, Giselle Anahí ; Bertrand, Lidwina ; Blettler, Martin Cesar Maria ; Buria, Leonardo Mario ; Cochero, Joaquin ; Di Prinzio, Cecilia Yanina ; Diaz, Boris Gastón; Díaz, Yamila; Diodato, Soledad Lorena ; Echaniz, Santiago Andrés; Espino Penilla, Marisol; Fasanella, Mariana ; Fernandez, Marina Olga ; Fontanarrosa, María Soledad ; Franco, Anahí Mailén; Franceschini, Maria Celeste ; Gastón, María Soledad ; Giorgi, Adonis David Nazareno ; Granitto, María ; Gultemirian, Maria de Lourdes ; Guzmán, Atilio Emiliano; Kuroda, Jorge A.; Macchi, Pablo Antonio; Mora, Guillermo Alejandro ; Nieto Peñalver, María Carolina ; Novarese, Pamela D.; Ossana, Natalia Alejandra ; Padulles, Maria Luz ; Pazos, Rocío Soledad ; Pecile, Maria Agostina ; Reissig, Mariana ; Reynaga, Maria Celina ; Rimondino, Guido Noé ; Ríos, Juan Manuel; Rocha, Luciana ; Rodriguez, Patricia Laura ; Rodriguez Castro, Maria Carolina ; Sabater, Lara Milena ; Schenone, Luca ; Seitz, Carina ; Tagliaferro, Marina Beatriz ; Herbert, Lucila Thomsett ; Vaira, Marcos ; Vignatti, Alicia María; Yacelga Villavicencio, Naomi Carolina (Wiley, 2024-04)
-
Artículo A poor and delayed anti-SARS-CoV2 IgG response is associated to severe COVID-19 in childrenSananez, Inés ; Raiden, Silvina Claudia ; Alghieri, Silvia C.; Urranga, Macarena; Grisolía, Nicolás; Filipo, Daniela; De Carli, Norberto; Di Lalla, Sandra; Cairoli, Nestor; Chiolo, Maria; Meregalli, Claudia; Cohen, Emilia; Mosquera, Gabriela; Marcó del Pont, Celia María; Gimenez, Lorena; Gregorio, Gabriela; Sarli, Mariam; Alcalde, Ana; Davenport, Carolina; Bruera, Maria; Simaz, Nancy; Pérez, Mariela F.; Nivela, Mariela; Bayle, Carola; Álvarez, Laura; Revetria, María; Tuccillo, Patricia; Agosta, María T.; Pérez, Hernán; Villa Nova, Susana; Suarez, Patricia; Takata, Eugenia M.; García, Mariela; Lattner, Jorge; Rolon, Maria Jose; Coll, Patricia; Salvatori, Melina ; Piccardo, Claudio; Russo, Constanza; Varese, Augusto ; Seery, Vanesa ; Holgado, María Pía ; Polo, Maria Laura ; Ceballos, Ana ; Nuñez, Myriam; Ferrero, Fernando; Geffner, Jorge Raúl ; Arruvito, Maria Lourdes (Elsevier, 2021-10)