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Artículo

Antioxidant effect of Cannabis sativa L. resin associated with Larrea divaricata Cav. extract: synergism, additivity and antagonism

Saint Martin, Elina MalénIcon ; Marrassini, CarlaIcon ; Silva Sofrás, Fresia MelinaIcon ; Peralta, Ignacio NahuelIcon ; Cogoi, Laura CarolinaIcon ; Van Baren, Catalina Maria; Alonso, María R.; Anesini, Claudia AlejandraIcon
Fecha de publicación: 12/2022
Editorial: Global Research Online
Revista: International Journal of Pharmaceutical Sciences Review and Research
ISSN: 0976-044X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Otras Ciencias de la Salud

Resumen

Cannabis sativa L. (Cannabaceae) is used as anticonvulsant in refractory epilepsy. Convulsions induce oxidative stress in the brain.Synergistic interactions between plants could improve the therapeutic and reduce adverse effects. Larrea divaricata Cav.’s(Zygophylliaceae) antioxidant activity is well-documented. The aim of this work was to evaluate the antioxidant activity of an ethanolicextract of C. sativa (CSR), study cannabidiol’s (CBD) participation and evaluate the possible synergistic effect of CSR with an aqueousextract of L. divaricata (LE). Antioxidant activity was determined by the 1,1-diphenyl-2-picryhydrazyl (DPPH) and the lipid peroxidationassays. For the DPPH assay, the inhibitory concentration 50 (IC50) were: CSR=19.82 ±1.47 µg/ml, LE=24.18±0.54µg/ml,CBD=12.49 ± 0.79µg/ml. The IC50 values for the combinations: CSR + LE 25 µg/ml=2.91±0.10µg/ml (p<0.001), CBD + LE 25µg/ml=4.19±0.11µg/ml (p<0.01). Combination indexes (CI) were: 15 µg/ml CSR / 25 µg/ml LE=0.38 (significant synergism), 10 µg/mlCBD / 25 µg/ml LE=0.39 (significant synergism). IC50 values for the lipid peroxidation assay: CSR=30.50±3.00µg/ml,LE=630.95±63.00µg/ml, CBD=10.20±1.00µg/ml. The IC50 values of the best combinations: CSR + 500 µg/ml LE=2.39±0.10µg/ml (p<0.0001), CBD + 500 µg/ml LE=2.19±0.18 µg/ml (p<0.0001); CI values: 10 µg/ml CSR /500 µg/ml LE=0.16 (strong synergism), 3 µg/mlCSR /500 µg/ml LE=0.36 (significant synergism). Conclusions: CBD is involved in CSR antioxidant activity. LE acted synergistically withCSR in both assays. CSR-LE association at optimal concentrations could be a good option to reach a significant synergism (CI<1). Atlow doses, the combination of CSR and LE could be used as co-adjuvant for the treatment of epilepsy to manage oxidative stress.
Palabras clave: CANNABIS SATIVA , LARREA DIVARICATA , ANTIOXIDANT ACTIVITY , COMBINATION INDEX
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/214647
URL: http://globalresearchonline.net/ijpsrr/v77-2/01.pdf
DOI: http://dx.doi.org/10.47583/ijpsrr.2022.v77i02.001
Colecciones
Articulos(IQUIMEFA)
Articulos de INST.QUIMICA Y METABOLISMO DEL FARMACO (I)
Citación
Saint Martin, Elina Malén; Marrassini, Carla; Silva Sofrás, Fresia Melina; Peralta, Ignacio Nahuel; Cogoi, Laura Carolina; et al.; Antioxidant effect of Cannabis sativa L. resin associated with Larrea divaricata Cav. extract: synergism, additivity and antagonism; Global Research Online; International Journal of Pharmaceutical Sciences Review and Research; 77; 2; 12-2022; 1-10
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