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dc.contributor.author
Martín Caballero, Lucía
dc.contributor.author
Capella, Matias
dc.contributor.author
Ramos Barrales, Ramón
dc.contributor.author
Dobrev, Nikolay
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van Emden, Thomas
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Hirano, Yasuhiro
dc.contributor.author
Suma Sreechakram, Vishnu N.
dc.contributor.author
Fischer Burkart, Sabine
dc.contributor.author
Kinugasa, Yasuha
dc.contributor.author
Nevers, Alicia
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Rougemaille, Mathieu
dc.contributor.author
Sinning, Irmgard
dc.contributor.author
Fischer, Tamás
dc.contributor.author
Hiraoka, Yasushi
dc.contributor.author
Braun, Sigurd
dc.date.available
2023-10-02T12:50:23Z
dc.date.issued
2022-09
dc.identifier.citation
Martín Caballero, Lucía; Capella, Matias; Ramos Barrales, Ramón; Dobrev, Nikolay; van Emden, Thomas; et al.; The inner nuclear membrane protein Lem2 coordinates RNA degradation at the nuclear periphery; Nature; Nature Structural and Molecular Biology; 29; 9; 9-2022; 910-921
dc.identifier.issn
1545-9993
dc.identifier.uri
http://hdl.handle.net/11336/213728
dc.description.abstract
Transcriptionally silent chromatin often localizes to the nuclear periphery. However, whether the nuclear envelope (NE) is a site for post-transcriptional gene repression is not well understood. Here we demonstrate that Schizosaccharomycespombe Lem2, an NE protein, regulates nuclear-exosome-mediated RNA degradation. Lem2 deletion causes accumulation of RNA precursors and meiotic transcripts and de-localization of an engineered exosome substrate from the nuclear periphery. Lem2 does not directly bind RNA but instead interacts with the exosome-targeting MTREC complex and its human homolog PAXT to promote RNA recruitment. This pathway acts largely independently of nuclear bodies where exosome factors assemble. Nutrient availability modulates Lem2 regulation of meiotic transcripts, implying that this pathway is environmentally responsive. Our work reveals that multiple spatially distinct degradation pathways exist. Among these, Lem2 coordinates RNA surveillance of meiotic transcripts and non-coding RNAs by recruiting exosome co-factors to the nuclear periphery.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
Long non-coding RNAs
dc.subject
Nuclear envelope
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RNA decay
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
The inner nuclear membrane protein Lem2 coordinates RNA degradation at the nuclear periphery
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-07T20:56:55Z
dc.identifier.eissn
1545-9985
dc.journal.volume
29
dc.journal.number
9
dc.journal.pagination
910-921
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Martín Caballero, Lucía. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Capella, Matias. Ludwig Maximilians Universitat; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Ramos Barrales, Ramón. Consejo Superior de Investigaciones Científicas; España. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Dobrev, Nikolay. Ruprecht Karls Universitat Heidelberg; Alemania
dc.description.fil
Fil: van Emden, Thomas. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Hirano, Yasuhiro. Osaka University; Japón
dc.description.fil
Fil: Suma Sreechakram, Vishnu N.. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Fischer Burkart, Sabine. Ludwig Maximilians Universitat; Alemania
dc.description.fil
Fil: Kinugasa, Yasuha. Osaka University; Japón
dc.description.fil
Fil: Nevers, Alicia. Universite Paris-Saclay;
dc.description.fil
Fil: Rougemaille, Mathieu. Universite Paris-Saclay;
dc.description.fil
Fil: Sinning, Irmgard. Ruprecht Karls Universitat Heidelberg; Alemania
dc.description.fil
Fil: Fischer, Tamás. Ruprecht Karls Universitat Heidelberg; Alemania
dc.description.fil
Fil: Hiraoka, Yasushi. Osaka University; Japón
dc.description.fil
Fil: Braun, Sigurd. Ludwig Maximilians Universitat; Alemania
dc.journal.title
Nature Structural and Molecular Biology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41594-022-00831-6
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