Artículo
The inner nuclear membrane protein Lem2 coordinates RNA degradation at the nuclear periphery
Martín Caballero, Lucía; Capella, Matias
; Ramos Barrales, Ramón; Dobrev, Nikolay; van Emden, Thomas; Hirano, Yasuhiro; Suma Sreechakram, Vishnu N.; Fischer Burkart, Sabine; Kinugasa, Yasuha; Nevers, Alicia; Rougemaille, Mathieu; Sinning, Irmgard; Fischer, Tamás; Hiraoka, Yasushi; Braun, Sigurd
Fecha de publicación:
09/2022
Editorial:
Nature
Revista:
Nature Structural and Molecular Biology
ISSN:
1545-9993
e-ISSN:
1545-9985
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Transcriptionally silent chromatin often localizes to the nuclear periphery. However, whether the nuclear envelope (NE) is a site for post-transcriptional gene repression is not well understood. Here we demonstrate that Schizosaccharomycespombe Lem2, an NE protein, regulates nuclear-exosome-mediated RNA degradation. Lem2 deletion causes accumulation of RNA precursors and meiotic transcripts and de-localization of an engineered exosome substrate from the nuclear periphery. Lem2 does not directly bind RNA but instead interacts with the exosome-targeting MTREC complex and its human homolog PAXT to promote RNA recruitment. This pathway acts largely independently of nuclear bodies where exosome factors assemble. Nutrient availability modulates Lem2 regulation of meiotic transcripts, implying that this pathway is environmentally responsive. Our work reveals that multiple spatially distinct degradation pathways exist. Among these, Lem2 coordinates RNA surveillance of meiotic transcripts and non-coding RNAs by recruiting exosome co-factors to the nuclear periphery.
Palabras clave:
Long non-coding RNAs
,
Nuclear envelope
,
RNA decay
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
Martín Caballero, Lucía; Capella, Matias; Ramos Barrales, Ramón; Dobrev, Nikolay; van Emden, Thomas; et al.; The inner nuclear membrane protein Lem2 coordinates RNA degradation at the nuclear periphery; Nature; Nature Structural and Molecular Biology; 29; 9; 9-2022; 910-921
Compartir
Altmétricas