Artículo
Functional specialization of the plant miR396 regulatory network through distinct microRNA-target interactions
Debernardi, Juan Manuel
; Rodriguez Virasoro, Ramiro Esteban
; Mecchia, Martin Alejandro
; Palatnik, Javier Fernando
Fecha de publicación:
01/2012
Editorial:
Public Library Of Science
Revista:
Plos Genetics
ISSN:
1553-7390
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
MicroRNAs (miRNAs) are ,21 nt small RNAs that regulate gene expression in animals and plants. They can be grouped into families comprising different genes encoding similar or identical mature miRNAs. Several miRNA families are deeply conserved in plant lineages and regulate key aspects of plant development, hormone signaling, and stress response. The ancient miRNA miR396 regulates conserved targets belonging to the GROWTH-REGULATING FACTOR (GRF) family of transcription factors, which are known to control cell proliferation in Arabidopsis leaves. In this work, we characterized the regulation of an additional target for miR396, the transcription factor bHLH74, that is necessary for Arabidopsis normal development. bHLH74 homologs with a miR396 target site could only be detected in the sister families Brassicaceae and Cleomaceae. Still, bHLH74 repression by miR396 is required for margin and vein pattern formation of Arabidopsis leaves. MiR396 contributes to the spatio-temporal regulation of GRF and bHLH74 expression during leaf development. Furthermore, a survey of miR396 sequences in different species showed variations in the 59 portion of the miRNA, a region known to be important for miRNA activity. Analysis of different miR396 variants in Arabidopsis thaliana revealed that they have an enhanced activity toward GRF transcription factors. The interaction between the GRF target site and miR396 has a bulge between positions 7 and 8 of the miRNA. Our data indicate that such bulge modulates the strength of the miR396-mediated repression and that this modulation is essential to shape the precise spatio-temporal pattern of GRF2 expression. The results show that ancient miRNAs can regulate conserved targets with varied efficiency in different species, and we further propose that they could acquire new targets whose control might also be biologically relevant.
Palabras clave:
Microrna
,
Evolution
,
Mir396
,
Function
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBR)
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Articulos de INST.DE BIOLOGIA MOLECULAR Y CELULAR DE ROSARIO
Citación
Debernardi, Juan Manuel; Rodriguez Virasoro, Ramiro Esteban; Mecchia, Martin Alejandro; Palatnik, Javier Fernando; Functional specialization of the plant miR396 regulatory network through distinct microRNA-target interactions; Public Library Of Science; Plos Genetics; 8; 1; 1-2012; 1-14; e1002419
Compartir
Altmétricas