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Artículo

Microfluidic Platform Integrated with Carbon Nanofibers-Decorated Gold Nanoporous Sensing Device for Serum PSA Quantification

Felici, Carlos EmilianoIcon ; Regiart, Daniel Matias GastonIcon ; Pereira, Sirley VanesaIcon ; Ortega, Francisco G.; Angnes, Lúcio; Messina, Germán AlejandroIcon ; Fernández Baldo, Martín AlejandroIcon
Fecha de publicación: 03/2023
Editorial: Multidisciplinary Digital Publishing Institute
Revista: Biosensors
ISSN: 2079-6374
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Química Analítica; Nano-materiales

Resumen

Prostate cancer is a disease with a high incidence and mortality rate in men worldwide. Serum prostate-specific antigens (PSA) are the main circulating biomarker for this disease in clinical practices. In this work, we present a portable and reusable microfluidic device for PSA quantification. This device comprises a polymethyl methacrylate microfluidic platform coupled with electrochemical detection. The platinum working microelectrode was positioned in the outflow region of the microchannel and was modified with carbon nanofibers (CNF)-decorated gold nanoporous (GNP) structures by the dynamic hydrogen bubble template method, through the simultaneous electrodeposition of metal precursors in the presence of CNF. CNF/GNP structures exhibit attractive properties, such as a large surface to volume ratio, which increases the antibody’s immobilization capacity and the electroactive area. CNFs/GNP structures were characterized by scanning electron microscopy, energy dispersive spectrometry, and cyclic voltammetry. Anti-PSA antibodies and HRP were employed for the immune-electrochemical reaction. The detection limit for the device was 5 pg mL−1, with a linear range from 0.01 to 50 ng mL−1. The coefficients of variation within and between assays were lower than 4.40%, and 6.15%, respectively. Additionally, its clinical performance was tested in serum from 30 prostate cancer patients. This novel device was a sensitive, selective, portable, and reusable tool for the serological diagnosis and monitoring of prostate cancer.
Palabras clave: CANCER BIOMARKER , CARBON NANOFIBERS , ELECTROCHEMICAL , GOLD NANOPOROUS , IMMUNOSENSOR , MICROFLUIDIC
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/208535
URL: https://www.mdpi.com/2079-6374/13/3/390
DOI: http://dx.doi.org/10.3390/bios13030390
Colecciones
Articulos(INQUISAL)
Articulos de INST. DE QUIMICA DE SAN LUIS
Citación
Felici, Carlos Emiliano; Regiart, Daniel Matias Gaston; Pereira, Sirley Vanesa; Ortega, Francisco G.; Angnes, Lúcio; et al.; Microfluidic Platform Integrated with Carbon Nanofibers-Decorated Gold Nanoporous Sensing Device for Serum PSA Quantification; Multidisciplinary Digital Publishing Institute; Biosensors; 13; 3; 3-2023; 390-403
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