Mostrar el registro sencillo del ítem

dc.contributor.author
Hyde, James
dc.contributor.author
Kezunovic, Nebojsa
dc.contributor.author
Urbano Suarez, Francisco Jose
dc.contributor.author
Garcia Rill, Edgar
dc.date.available
2017-07-13T18:30:28Z
dc.date.issued
2013-04
dc.identifier.citation
Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Visualization of fast calcium oscillations in the parafascicular nucleus; Springer; Pflugers Archiv-european Journal Of Physiology; 465; 9; 4-2013; 1327-1340
dc.identifier.issn
0031-6768
dc.identifier.uri
http://hdl.handle.net/11336/20364
dc.description.abstract
The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arousal
dc.subject
Gamma Band Activity
dc.subject
Intralaminar Thalamus
dc.subject
Parafascicular
dc.subject
P/Q Type Calcium Channels
dc.subject
N Type Calcium Channels
dc.subject.classification
Neurociencias
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Visualization of fast calcium oscillations in the parafascicular nucleus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-11T13:25:09Z
dc.identifier.eissn
1432-2013
dc.journal.volume
465
dc.journal.number
9
dc.journal.pagination
1327-1340
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Hyde, James. University Of Arkansas For Medical Sciences; Estados Unidos
dc.description.fil
Fil: Kezunovic, Nebojsa. University Of Arkansas For Medical Sciences; Estados Unidos
dc.description.fil
Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.description.fil
Fil: Garcia Rill, Edgar. University Of Arkansas For Medical Sciences; Estados Unidos
dc.journal.title
Pflugers Archiv-european Journal Of Physiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00424-013-1264-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00424-013-1264-6
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748156/


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem