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dc.contributor.author Hyde, James
dc.contributor.author Kezunovic, Nebojsa
dc.contributor.author Urbano Suarez, Francisco Jose
dc.contributor.author Garcia Rill, Edgar
dc.date.available 2017-07-13T18:30:28Z
dc.date.issued 2013-04
dc.identifier.citation Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Visualization of fast calcium oscillations in the parafascicular nucleus; Springer; Pflugers Archiv-european Journal Of Physiology; 465; 9; 4-2013; 1327-1340
dc.identifier.issn 0031-6768
dc.identifier.uri http://hdl.handle.net/11336/20364
dc.description.abstract The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.
dc.format application/pdf
dc.language.iso eng
dc.publisher Springer
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject AROUSAL
dc.subject GAMMA BAND ACTIVITY
dc.subject INTRALAMINAR THALAMUS
dc.subject PARAFASCICULAR
dc.subject P/Q TYPE CALCIUM CHANNELS
dc.subject N TYPE CALCIUM CHANNELS
dc.subject.classification Neurociencias
dc.subject.classification Medicina Básica
dc.subject.classification CIENCIAS MÉDICAS Y DE LA SALUD
dc.title Visualization of fast calcium oscillations in the parafascicular nucleus
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-11T13:25:09Z
dc.identifier.eissn 1432-2013
dc.journal.volume 465
dc.journal.number 9
dc.journal.pagination 1327-1340
dc.journal.pais Alemania
dc.journal.ciudad Berlin
dc.description.fil Fil: Hyde, James. University Of Arkansas For Medical Sciences; Estados Unidos
dc.description.fil Fil: Kezunovic, Nebojsa. University Of Arkansas For Medical Sciences; Estados Unidos
dc.description.fil Fil: Urbano Suarez, Francisco Jose. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Fisiología, Biología Molecular y Neurociencias. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
dc.description.fil Fil: Garcia Rill, Edgar. University Of Arkansas For Medical Sciences; Estados Unidos
dc.journal.title Pflugers Archiv-european Journal Of Physiology
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00424-013-1264-6
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00424-013-1264-6
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748156/


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)