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Artículo

Visualization of fast calcium oscillations in the parafascicular nucleus

Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco JoseIcon ; Garcia Rill, Edgar
Fecha de publicación: 04/2013
Editorial: Springer
Revista: Pflugers Archiv-european Journal Of Physiology
ISSN: 0031-6768
e-ISSN: 1432-2013
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Neurociencias

Resumen

The parafascicular nucleus (Pf) is an ascending target of the pedunculopontine nucleus (PPN) and is part of the “non-specific” intralaminar thalamus. The PPN, part of the reticular activating system, is mainly involved in waking and rapid eye movement sleep. Gamma oscillations are evident in all Pf neurons and mediated by high threshold voltage-dependent N- and P/Q-type calcium channels. We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in synchrony with patch clamp recorded oscillations during depolarizing current ramps. Patch-clamped 9 to 19-day-old rat Pf neurons (n = 148, dye filled n = 61, control n = 87) were filled with Fura 2, Bis Fura, or Oregon Green BAPTA-1. Calcium transients were generated during depolarizing current ramps and visualized with a high-speed, wide-field fluorescence imaging system. Cells manifested calcium transients with oscillations in both somatic and proximal dendrite fluorescence recordings. Fluorescent calcium transients were blocked with the nonspecific calcium channel blocker, cadmium, or the combination of ω-Agatoxin-IVA (AgA), a specific P/Q-type calcium channel blocker and ω-conotoxin-GVIA (CgTx), a specific N-type calcium channel blocker. We developed a viable methodology for studying high-speed oscillations without the use of multi-photon imaging systems.
Palabras clave: Arousal , Gamma Band Activity , Intralaminar Thalamus , Parafascicular , P/Q Type Calcium Channels , N Type Calcium Channels
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/20364
DOI: http://dx.doi.org/10.1007/s00424-013-1264-6
URL: https://link.springer.com/article/10.1007%2Fs00424-013-1264-6
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3748156/
Colecciones
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Hyde, James; Kezunovic, Nebojsa; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Visualization of fast calcium oscillations in the parafascicular nucleus; Springer; Pflugers Archiv-european Journal Of Physiology; 465; 9; 4-2013; 1327-1340
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