Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss

Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; Cardillo, Alejandra Beatriz; Palazon, Javier

doi:10.1007/s00044-012-0067-1

Artículo

Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss

Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; Cardillo, Alejandra Beatriz Icon

; Palazon, Javier

Fecha de publicación: 02/2013

Editorial: Birkhauser Boston Inc

Revista: Medicinal Chemistry Research

ISSN: 1054-2523

e-ISSN: 1554-8120

Idioma: Inglés

Tipo de recurso: Artículo publicado

Clasificación temática:

Biología Celular, Microbiología; Bioproductos, Biomateriales, Bioplásticos, Biocombustibles, Bioderivados, etc.; Farmacología y Farmacia

Resumen

Linum album is a herbaceous medicinal plant that contains some lignans with antiviral and anticancer properties such as podophyllotoxin (PTOX) and 6-methoxy podophyllotoxin (MPTOX). In this research, hairy root cultures of L. album were established by transformation with Agrobacterium rhizogenes strains LBA9402, A4, AR15834 and A. tumefaciens strain C58C1 (pRiA4). The presence of PTOX and MPTOX in the hairy roots was verified by ESI/MS in positive ion mode. MPTOX was confirmed and its enantiomer determined by nuclear magnetic resonance spectroscopy and circular dichroism spectroscopy, respectively. PTOX and MPTOX production was determined by HPLC, in different lines of hairy roots. The results showed that all obtained hairy root lines produced higher yield of lignans than mother plant roots. In addition, the lignans content in the roots derived from A. rhizogenes strain LBA9402 was higher than in those obtained from A. tumefaciens strain C58C1.

Palabras clave: 6-METHOXY PODOPHYLLOTOXIN (MPTOX) , AGROBACTERIUM , HAIRY ROOTS , LINUM ALBUM , PODOPHYLLOTOXIN (PTOX)

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Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)

Identificadores

URI: http://hdl.handle.net/11336/1934

URL: http://link.springer.com/article/10.1007%2Fs00044-012-0067-1

DOI: http://dx.doi.org/10.1007/s00044-012-0067-1

Colecciones

Articulos(NANOBIOTEC)
Articulos de INSTITUTO DE NANOBIOTECNOLOGIA

Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY

Citación

Ahmadian Chashmi, Najmeh; Sharifi, Mozafar; Yousefzadi, Morteza; Behmanesh, Mehrdad; Rezadoost, Hassan; et al.; Analysis of 6-methoxy podophyllotoxin and podophyllotoxin in hairy root cultures of Linum album Kotschy ex Boiss; Birkhauser Boston Inc; Medicinal Chemistry Research; 22; 2; 2-2013; 745-752

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