Mostrar el registro sencillo del ítem
dc.contributor.author
Santos Lacerda, Thaís Lourdes
dc.contributor.author
Gomes Cardoso, Patrícia
dc.contributor.author
De Almeida, Leonardo Augusto
dc.contributor.author
Lopes Baratella da Cunha Camargo, Ilana
dc.contributor.author
Almeida Freitas Afonso, Daniela
dc.contributor.author
Cardoso Trant, Cyntia
dc.contributor.author
Costa Macedo, Gilson
dc.contributor.author
Campos, Eleonora
dc.contributor.author
Cravero, Silvio L.
dc.contributor.author
Salcedo, Suzana P.
dc.contributor.author
Gorvel, Jean Pierre
dc.contributor.author
Costa Oliveira, Sérgio
dc.date.available
2023-03-22T11:30:57Z
dc.date.issued
2010-10
dc.identifier.citation
Santos Lacerda, Thaís Lourdes; Gomes Cardoso, Patrícia; De Almeida, Leonardo Augusto; Lopes Baratella da Cunha Camargo, Ilana; Almeida Freitas Afonso, Daniela; et al.; Inactivation of formyltransferase (wbkC) gene generates an attenuated 1 Brucella abortus 2 rough strain that is attenuated in macrophages and in mice; Elsevier; Vaccine; 28; 10-2010; 5627-5634
dc.identifier.issn
0264-410X
dc.identifier.uri
http://hdl.handle.net/11336/191316
dc.description.abstract
Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. DeltawbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
BRUCELLA ABORTUS
dc.subject
ROUGH STARIN
dc.subject
LIVE VACCINE
dc.subject.classification
Otras Biotecnología Agropecuaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Inactivation of formyltransferase (wbkC) gene generates an attenuated 1 Brucella abortus 2 rough strain that is attenuated in macrophages and in mice
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-03-15T10:17:54Z
dc.journal.volume
28
dc.journal.pagination
5627-5634
dc.journal.pais
Países Bajos
dc.description.fil
Fil: Santos Lacerda, Thaís Lourdes. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Gomes Cardoso, Patrícia. Universidad Federal de Lavras; Brasil
dc.description.fil
Fil: De Almeida, Leonardo Augusto. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Lopes Baratella da Cunha Camargo, Ilana. Universidade de Sao Paulo; Brasil
dc.description.fil
Fil: Almeida Freitas Afonso, Daniela. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Cardoso Trant, Cyntia. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Costa Macedo, Gilson. Universidade Federal de Minas Gerais; Brasil
dc.description.fil
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Cravero, Silvio L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
dc.description.fil
Fil: Salcedo, Suzana P.. Inserm; Francia
dc.description.fil
Fil: Gorvel, Jean Pierre. Inserm; Francia
dc.description.fil
Fil: Costa Oliveira, Sérgio. Universidade Federal de Minas Gerais; Brasil
dc.journal.title
Vaccine
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0264410X10008236
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vaccine.2010.06.023
Archivos asociados
Tamaño:
3.409Mb
Formato:
PDF