Artículo

Inactivation of formyltransferase (wbkC) gene generates an attenuated 1 Brucella abortus 2 rough strain that is attenuated in macrophages and in mice

Santos Lacerda, Thaís Lourdes; Gomes Cardoso, Patrícia; De Almeida, Leonardo Augusto; Lopes Baratella da Cunha Camargo, Ilana; Almeida Freitas Afonso, Daniela; Cardoso Trant, Cyntia; Costa Macedo, Gilson; Campos, EleonoraIcon ; Cravero, Silvio L.; Salcedo, Suzana P.; Gorvel, Jean Pierre; Costa Oliveira, Sérgio
Fecha de publicación: 10/2010
Editorial: Elsevier
Revista: Vaccine
ISSN: 0264-410X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:

Resumen

Rough mutants of Brucella abortus were generated by disruption of wbkC gene which encodes the formyltransferase enzyme involved in LPS biosynthesis. In bone marrow-derived macrophages the B. abortusDeltawbkC mutants were attenuated, could not reach a replicative niche and induced higher levels of IL-12 and TNF-alpha when compared to parental smooth strains. Additionally, mutants exhibited attenuation in vivo in C57BL/6 and interferon regulatory factor-1 knockout mice. DeltawbkC mutant strains induced lower protective immunity in C56BL/6 than smooth vaccine S19 but similar to rough vaccine RB51. Finally, we demonstrated that Brucella wbkC is critical for LPS biosynthesis and full bacterial virulence.
Palabras clave: BRUCELLA ABORTUS , ROUGH STARIN , LIVE VACCINE
 
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/191316
URL: https://www.sciencedirect.com/science/article/pii/S0264410X10008236
DOI: http://dx.doi.org/10.1016/j.vaccine.2010.06.023
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Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Citación
Santos Lacerda, Thaís Lourdes; Gomes Cardoso, Patrícia; De Almeida, Leonardo Augusto; Lopes Baratella da Cunha Camargo, Ilana; Almeida Freitas Afonso, Daniela; et al.; Inactivation of formyltransferase (wbkC) gene generates an attenuated 1 Brucella abortus 2 rough strain that is attenuated in macrophages and in mice; Elsevier; Vaccine; 28; 10-2010; 5627-5634
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