Mostrar el registro sencillo del ítem

dc.contributor.author
Raffo, Diego Alejandro
dc.contributor.author
Berardi, Damian Emilio
dc.contributor.author
Pontiggia, Osvaldo Alejandro
dc.contributor.author
Todaro, Laura Beatriz
dc.contributor.author
Bal, Elisa Dora
dc.contributor.author
Simian, Marina
dc.date.available
2015-09-02T14:37:40Z
dc.date.issued
2013-12
dc.identifier.citation
Raffo, Diego Alejandro; Berardi, Damian Emilio; Pontiggia, Osvaldo Alejandro; Todaro, Laura Beatriz; Bal, Elisa Dora; et al.; Tamoxifen selects for breast cancer cells with mammosphere forming capacity and increased growth rate.; Springer; Breast Cancer Research and Treatment; 142; 3; 12-2013; 537-548
dc.identifier.issn
0167-6806
dc.identifier.issn
http://dx.doi.org/10.1007/s10549-013-2760-2
dc.identifier.uri
http://hdl.handle.net/11336/1896
dc.description.abstract
Using the M05 mouse mammary tumor model and the MCF-7 cell line, we investigated the effect of tamoxifen treatment on the fraction of breast cancer cells with self-renewing capacity both in vitro and in vivo. We found that pretreatment with 4-OH-tamoxifen leads to an increase in cells with the ability of forming mammospheres that express lower levels of ER-α and increased expression of transcription factors associated with pluripotency. Moreover, exposure on plastic to 4-OH-tamoxifen by itself leads to an upregulation of these transcription factors. M05 tumors grown in mice treated with tamoxifen have a higher percentage of cells with self-renewing capacity and this proportion is conserved when tumors are passaged to nontreated mice. Furthermore, interruption of tamoxifen leads to increased tumor growth compared to tumors grown in mice that were never exposed to the antiestrogen. In addition, these tumors are characterized by a higher number of CD24(l)CD29(h) cells compared to tumors grown in nontreated mice. Treatment in vitro with 4-OH-tamoxifen for 5 days leads to a long lasting increase in the proportion of cells with self-renewing capacity even after 1 month of growth in the absence of the antiestrogen. Finally, we compared the mammosphere forming capacity of hormone dependent and independent passages of the M05 tumor and found that hormone independence is associated to an increase in cells with self-renewing capacity. Our results support previous findings that suggest that endocrine treatment selects for cells with stem cell properties.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Breast Cancer
dc.subject
Tamoxifen
dc.subject
Stem Cells
dc.subject.classification
Farmacología y Farmacia
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.subject.classification
Oncología
dc.subject.classification
Medicina Clínica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Tamoxifen selects for breast cancer cells with mammosphere forming capacity and increased growth rate.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.identifier.eissn
1573-7217
dc.journal.volume
142
dc.journal.number
3
dc.journal.pagination
537-548
dc.journal.pais
Estados Unidos de América
dc.journal.ciudad
Nueva York
dc.description.fil
Fil: Raffo, Diego Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
dc.description.fil
Fil: Berardi, Damian Emilio. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
dc.description.fil
Fil: Pontiggia, Osvaldo Alejandro. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
dc.description.fil
Fil: Todaro, Laura Beatriz. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
dc.description.fil
Fil: Bal, Elisa Dora. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
dc.description.fil
Fil: Simian, Marina. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Oncología; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina;
dc.journal.title
Breast Cancer Research and Treatment
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007%2Fs10549-013-2760-2


Archivos asociados

Documento no disponible

Comunidades y colecciones

Mostrar el registro sencillo del ítem