Artículo
Tamoxifen selects for breast cancer cells with mammosphere forming capacity and increased growth rate
Raffo, Diego Alejandro
; Berardi, Damian Emilio
; Pontiggia, Osvaldo Alejandro
; Todaro, Laura Beatriz
; Bal, Elisa Dora
; Simian, Marina
Fecha de publicación:
12/2013
Editorial:
Springer
Revista:
Breast Cancer Research and Treatment
ISSN:
0167-6806
e-ISSN:
1573-7217
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Using the M05 mouse mammary tumor model and the MCF-7 cell line, we investigated the effect of tamoxifen treatment on the fraction of breast cancer cells with self-renewing capacity both in vitro and in vivo. We found that pretreatment with 4-OH-tamoxifen leads to an increase in cells with the ability of forming mammospheres that express lower levels of ER-α and increased expression of transcription factors associated with pluripotency. Moreover, exposure on plastic to 4-OH-tamoxifen by itself leads to an upregulation of these transcription factors. M05 tumors grown in mice treated with tamoxifen have a higher percentage of cells with self-renewing capacity and this proportion is conserved when tumors are passaged to nontreated mice. Furthermore, interruption of tamoxifen leads to increased tumor growth compared to tumors grown in mice that were never exposed to the antiestrogen. In addition, these tumors are characterized by a higher number of CD24(l)CD29(h) cells compared to tumors grown in nontreated mice. Treatment in vitro with 4-OH-tamoxifen for 5 days leads to a long lasting increase in the proportion of cells with self-renewing capacity even after 1 month of growth in the absence of the antiestrogen. Finally, we compared the mammosphere forming capacity of hormone dependent and independent passages of the M05 tumor and found that hormone independence is associated to an increase in cells with self-renewing capacity. Our results support previous findings that suggest that endocrine treatment selects for cells with stem cell properties.
Palabras clave:
Breast Cancer
,
Tamoxifen
,
Stem Cells
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Raffo, Diego Alejandro; Berardi, Damian Emilio; Pontiggia, Osvaldo Alejandro; Todaro, Laura Beatriz; Bal, Elisa Dora; et al.; Tamoxifen selects for breast cancer cells with mammosphere forming capacity and increased growth rate; Springer; Breast Cancer Research and Treatment; 142; 3; 12-2013; 537-548
Compartir
Altmétricas