Artículo
Characterization of blaKPC-2 harboring plasmids recovered from Pseudomonas aeruginosa ST654 and ST235 high-risk clones.
Cejas, Daniela
; Elena, Alan Xavier
; Gonzalez Espinosa, Francisco Eduardo
; Pallecchi, Lucia; Vay, Carlos; Rossolini, Gian Maria; Gutkind, Gabriel Osvaldo
; Di Pilato, Vincenzo; Radice, Marcela Alejandra
Fecha de publicación:
06/2022
Editorial:
Elsevier
Revista:
Journal of Global Antimicrobial Resistance
ISSN:
2213-7165
e-ISSN:
2213-7173
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Objective: The main objectives were to describe two blaKPC-2 plasmids recovered from Pseudomonas aeruginosa isolates belonging to the ST654 and ST235 high-risk clones, and to compare with complete sequences of blaKPC-2 harbouring plasmids available in public databases. Methods: Antimicrobial susceptibility was determined according to CLSI (Clinical and Laboratory Standards Institute) guidelines. Genomes were sequenced using an Illumina MiSeq platform, and blaKPC-2 plasmid sequences were achieved using MinION platform. Sequences were analysed using Unicycler and RAST. In silico predictions of the isolates sequence type (ST), antimicrobial resistance genes, plasmid replicon typing and MOB relaxases were fulfilled using bioinformatics tools. Results: PA_2047 and PA_HdC isolates corresponded to the high-risk clones ST654 and ST235, respectively. The carbapenem resistance was mediated by KPC-2. Both blaKPC-2 harbouring plasmids, pPA_2047 and pPA_HdC, were different among them, non-conjugative and untypable by PlasmidFinder. pPA_2047 presented high identity with a Pae-13 plasmid, and these both located blaKPC-2 in Tn4401b isoform. pPA_HdC displayed a novel architecture, and the genetic context of blaKPC-2 was original. Besides the blaKPC-2 gene, resistance genes to aminoglycosides and quinolones were detected, including the novel phosphotransferase CrpP in PA_HdC. Conclusion: This study expands the limited knowledge about the molecular epidemiology of blaKPC-2 in P. aeruginosa from Latin America. Two novel plasmids harbouring blaKPC-2 were described that were untypable by their incompatibility group. The plasmid recovered from P. aeruginosa PA_HdC (ST235) displayed a novel architecture and an original context for blaKPC-2. On the other hand, the genetic platform carrying blaKPC-2 in P. aeruginosa PA_2047 (ST654) seems to a be a classical one.
Palabras clave:
BLAKPC-2
,
PLASMIDS
,
PSEUDOMONAS AERUGINOSA
,
ST235
,
ST654
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Identificadores
Colecciones
Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Cejas, Daniela; Elena, Alan Xavier; Gonzalez Espinosa, Francisco Eduardo; Pallecchi, Lucia; Vay, Carlos; et al.; Characterization of blaKPC-2 harboring plasmids recovered from Pseudomonas aeruginosa ST654 and ST235 high-risk clones.; Elsevier; Journal of Global Antimicrobial Resistance; 29; 6-2022; 310-312
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