Artículo
Shelterin and subtelomeric DNA sequences control nucleosome maintenance and genome stability
van Emden, Thomas S.; Forn, Marta; Forné, Ignasi; Sarkadi, Zsuzsa; Capella, Matias
; Martín Caballero, Lucía; Fischer Burkart, Sabine; Brönner, Cornelia; Simonetta, Marco; Toczyski, David; Halic, Mario; Imhof, Axel; Braun, Sigurd
Fecha de publicación:
01/2019
Editorial:
Nature Publishing Group
Revista:
Embo Reports
ISSN:
1469-221X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Telomeres and the shelterin complex cap and protect the ends of chromosomes. Telomeres are flanked by the subtelomeric sequences that have also been implicated in telomere regulation, although their role is not well defined. Here, we show that, in Schizosaccharomyces pombe, the telomere-associated sequences (TAS) present on most subtelomeres are hyper-recombinogenic, have metastable nucleosomes, and unusual low levels of H3K9 methylation. Ccq1, a subunit of shelterin, protects TAS from nucleosome loss by recruiting the heterochromatic repressor complexes CLRC and SHREC, thereby linking nucleosome stability to gene silencing. Nucleosome instability at TAS is independent of telomeric repeats and can be transmitted to an intrachromosomal locus containing an ectopic TAS fragment, indicating that this is an intrinsic property of the underlying DNA sequence. When telomerase recruitment is compromised in cells lacking Ccq1, DNA sequences present in the TAS promote recombination between chromosomal ends, independent of nucleosome abundance, implying an active function of these sequences in telomere maintenance. We propose that Ccq1 and fragile subtelomeres co-evolved to regulate telomere plasticity by controlling nucleosome occupancy and genome stability.
Palabras clave:
GENOME STABILITY
,
HETEROCHROMATIN
,
NUCLEOSOMES
,
SHELTERIN
,
TELOMERES
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Articulos(IAL)
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Articulos de INSTITUTO DE AGROBIOTECNOLOGIA DEL LITORAL
Citación
van Emden, Thomas S.; Forn, Marta; Forné, Ignasi; Sarkadi, Zsuzsa; Capella, Matias; et al.; Shelterin and subtelomeric DNA sequences control nucleosome maintenance and genome stability; Nature Publishing Group; Embo Reports; 20; 1; 1-2019; 1-17
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