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dc.contributor.author
Niebling, Stephan
dc.contributor.author
Burastero, Osvaldo
dc.contributor.author
Bürgi, Jérôme
dc.contributor.author
Günther, Christian
dc.contributor.author
Defelipe, Lucas Alfredo
dc.contributor.author
Sander, Simon
dc.contributor.author
Gattkowski, Ellen
dc.contributor.author
Anjanappa, Raghavendra
dc.contributor.author
Wilmanns, Matthias
dc.contributor.author
Springer, Sebastian
dc.contributor.author
Tidow, Henning
dc.contributor.author
García Alai, María
dc.date.available
2022-12-27T10:50:51Z
dc.date.issued
2021-12
dc.identifier.citation
Niebling, Stephan; Burastero, Osvaldo; Bürgi, Jérôme; Günther, Christian; Defelipe, Lucas Alfredo; et al.; FoldAffinity: Binding affinities from nDSF experiments; Nature Publishing Group; Scientific Reports; 11; 1; 12-2021; 1-17
dc.identifier.uri
http://hdl.handle.net/11336/182430
dc.description.abstract
Differential scanning fluorimetry (DSF) using the inherent fluorescence of proteins (nDSF) is a popular technique to evaluate thermal protein stability in different conditions (e.g. buffer, pH). In many cases, ligand binding increases thermal stability of a protein and often this can be detected as a clear shift in nDSF experiments. Here, we evaluate binding affinity quantification based on thermal shifts. We present four protein systems with different binding affinity ligands, ranging from nM to high μM. Our study suggests that binding affinities determined by isothermal analysis are in better agreement with those from established biophysical techniques (ITC and MST) compared to apparent Kds obtained from melting temperatures. In addition, we describe a method to optionally fit the heat capacity change upon unfolding (Δ Cp) during the isothermal analysis. This publication includes the release of a web server for easy and accessible application of isothermal analysis to nDSF data.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Nature Publishing Group
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
NanoDSF
dc.subject
Isothermal Analysis
dc.subject
Affinity Determination
dc.subject.classification
Otras Ciencias Químicas
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Ciencias Químicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
FoldAffinity: Binding affinities from nDSF experiments
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-09-22T00:31:22Z
dc.identifier.eissn
2045-2322
dc.journal.volume
11
dc.journal.number
1
dc.journal.pagination
1-17
dc.journal.pais
Reino Unido
dc.journal.ciudad
Londres
dc.description.fil
Fil: Niebling, Stephan. Centre for Structural Systems Biology; Alemania. European Molecular Biology Laboratory; Alemania
dc.description.fil
Fil: Burastero, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. European Molecular Biology Laboratory; Alemania
dc.description.fil
Fil: Bürgi, Jérôme. European Molecular Biology Laboratory; Alemania
dc.description.fil
Fil: Günther, Christian. European Molecular Biology Laboratory; Alemania
dc.description.fil
Fil: Defelipe, Lucas Alfredo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. European Molecular Biology Laboratory; Alemania
dc.description.fil
Fil: Sander, Simon. Universitat Hamburg; Alemania
dc.description.fil
Fil: Gattkowski, Ellen. Universitat Hamburg; Alemania
dc.description.fil
Fil: Anjanappa, Raghavendra. Universitat Bremen. School of Engineering and Science Jacobs; Alemania
dc.description.fil
Fil: Wilmanns, Matthias. European Molecular Biology Laboratory; Alemania. Universitat Hamburg; Alemania
dc.description.fil
Fil: Springer, Sebastian. Universitat Bremen. School of Engineering and Science Jacobs; Alemania
dc.description.fil
Fil: Tidow, Henning. Universitat Hamburg; Alemania
dc.description.fil
Fil: García Alai, María. European Molecular Biology Laboratory; Alemania. Centre for Structural Systems Biology; Alemania
dc.journal.title
Scientific Reports
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.nature.com/articles/s41598-021-88985-z
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41598-021-88985-z
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