Artículo
Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina
Ricatti, Maria Jimena; Battista, Ariadna Gabriela
; Zorrilla Zubilete, María Aurelia
; Faillace, Maria Paula
Fecha de publicación:
04/2011
Editorial:
SAGE Publications
Revista:
Journal of Biological Rhythms
ISSN:
0748-7304
e-ISSN:
1552-4531
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Regeneration and growth that occur in the adult teleost retina have been helpful in identifying molecular and cellular mechanisms underlying cell proliferation and differentiation. Here, it is reported that S-phase cell number, in the ciliary marginal zone (CMZ) of the adult zebrafish retina, exhibits day-night variations with a mid-light phase peak. Oscillations persist for 24 h in constant darkness (DD), suggesting control by a circadian component. However, variations in the S-phase nuclei number were rapidly dampened and not present during and after a second day in DD. An ADPβS treatment significantly enhanced S-phase activity at night to mid-light levels, as assessed by in vivo BrdU incorporation in a 2-h interval. Moreover, daylight increase in S-phase cell number was completely abolished when extracellular nucleotide levels or their extracellular hydrolysis by ectonucleoside triphosphate diphosphohydrolases (NTPDases) were significantly disrupted or when a selective antagonist of purinergic P2Y1 receptors was intraocularly injected before BrdU exposure. Extracellular nucleotides and NTPDase action were also important for maintaining nocturnal low levels of S-phase activity in the CMZ. Finally, we showed that mRNAs of NTPDases 1, 2 (3 isoforms), and 3 as well as of P2Y1 receptor are present in the neural retina of zebrafish. NTPDase mRNA expression exhibited a 2-fold increment in light versus dark conditions as assessed by quantitative RT-PCR, whereas P2Y1 receptor mRNA levels did not show significant day-night variations. This study demonstrates a key role for nucleotides, principally ADP as a paracrine signal, as well as for NTPDases, the plasma membrane—bound enzymes that control extracellular nucleotide concentration, for inducing S-phase cell entry in the CMZ—normally associated with retinal growth—throughout the light-dark cycle.
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Articulos(IQUIFIB)
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Articulos de INST.DE QUIMICA Y FISICO-QUIMICA BIOLOGICAS "PROF. ALEJANDRO C. PALADINI"
Citación
Ricatti, Maria Jimena; Battista, Ariadna Gabriela; Zorrilla Zubilete, María Aurelia; Faillace, Maria Paula; Purinergic signals regulate daily s-phase cell activity in the ciliary marginal zone of the zebrafish retina; SAGE Publications; Journal of Biological Rhythms; 26; 2; 4-2011; 107-117
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