Artículo
Bioseparation of papain from Carica papaya latex by precipitation of papain- poly (vinyl sulfonate) complexes
Braia, Mauricio Javier
; Ferrero, Maximiliano; Rocha, María Victoria
; Loureiro, Dana Belen
; Tubio, Gisela
; Romanini, Diana
Fecha de publicación:
07/2013
Editorial:
Academic Press Inc Elsevier Science
Revista:
Protein Expression and Purification
ISSN:
1046-5928
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The formation of insoluble complexes between enzymes and polyelectrolytes is a suitable technique for isolating these biomolecules from natural sources, because it is a simple and rapid technique that allows the concentration of the protein. This technique can be used in most purification protocols at the beginning of the downstream process. The aim of this investigation is to isolate papain from Carica papaya latex by precipitation of insoluble complexes between this enzyme and poly (vinyl sulfonate). The papain-poly (vinyl sulfonate) complex was insoluble at pH lower than 6, with a PVS/PAP stoichiometric ratio of 1:279. Ionic strength affected the complex formation. The presence of the polymer increased the enzymatic activity and protected the enzyme from autodegradation. The optimal conditions for the formation of insoluble papain-polyelectrolyte complex formation were applied to C. papaya latex and a high recovery was obtained (around 86%) and a purification factor around 2. This method can be applied as an isolation method of papain from C. papaya latex or as a first step in a larger purification strategy.
Palabras clave:
BIOSEPARATION
,
PAPAIN
,
POLY (VINYL SULFONATE)
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Licencia
Identificadores
Colecciones
Articulos(CCT - ROSARIO)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - ROSARIO
Citación
Braia, Mauricio Javier; Ferrero, Maximiliano; Rocha, María Victoria; Loureiro, Dana Belen; Tubio, Gisela; et al.; Bioseparation of papain from Carica papaya latex by precipitation of papain- poly (vinyl sulfonate) complexes; Academic Press Inc Elsevier Science; Protein Expression and Purification; 91; 1; 7-2013; 91-95
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