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dc.contributor.author
Madanes, Daniela
dc.contributor.author
Meresman, Gabriela Fabiana
dc.contributor.author
Valla, Sofía Aylén
dc.contributor.author
Hassan, Nourhan
dc.contributor.author
Kiesel, Ludwig
dc.contributor.author
Greve, Burkhard
dc.contributor.author
Barañao, Rosa Ines
dc.contributor.author
Götte, Martin
dc.contributor.author
Ricci, Analía Gabriela
dc.date.available
2022-10-06T02:48:34Z
dc.date.issued
2022
dc.identifier.citation
Madanes, Daniela; Meresman, Gabriela Fabiana; Valla, Sofía Aylén; Hassan, Nourhan; Kiesel, Ludwig; et al.; Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis; Reproductive Healthcare Ltd; Reproductive Biomedicine Online; 44; 6; 2022; 976-990
dc.identifier.issn
1472-6483
dc.identifier.uri
http://hdl.handle.net/11336/172104
dc.description.abstract
Research question: Does resveratrol exert a potent inhibitory effect on the development of endometriosis by interfering with some pivotal processes?Design: In-vitro cultures of primary endometriotic stromal cells, immortalized endometrial stromal (St-T1b) and endometriotic epithelial (12Z) cells were used to assess the effects of resveratrol on endometrial cell mechanisms. The effects of resveratrol on 12Z and St-T1b cell viability were assessed by MTT assay, apoptosis by FITC Annexin V assay and cleaved caspase-3 levels and cell migration by wound healing assay. The effect of resveratrol on the expression of genes related to cell migration, angiogenesis and cell stemness was evaluated by qRT-PCR.Results: Resveratrol significantly decreased cell viability (P= 0.0065 to P = 0.0180), cell migration (P < 0.001 to P = 0.0225) and increased the number of apoptotic cells (P = 0.0031 to P = 0.0432) in both cell lines. In cell lines and primary culture, the treatment reduced MMP-2/TIMP-1 (P < 0.001 to P = 0.0180), VEGF (P = 0.0052 to P = 0.0243) and Ang-1 mRNA (P < 0.001 to P = 0.0382) expression. Among the stem cell phenotype markers, resveratrol 100 µM increased mRNA expression levels of Notch-1 (P < 0.001 to P = 0.0018), KLF-4 (P = 0.0011 to P = 0.0137), SOX-2 (P < 0.001 to P = 0.0070) and TERT (P < 0.001 to P = 0.0193) in both cell lines and primary cultures. The mRNA expression level of Snail-1 increased in the cell lines (P < 0.001 to P = 0.0087), whereas OCT-4 mRNA expression increased in St-T1b (P = 0.0396) and primary cultures (P = 0.0148). Vimentin mRNA expression showed a significant upregulation in primary cultures (P < 0.001). The expression of Msi-1 (P = 0.0145) and NANOG (P = 0.0080) decreased only in St-T1b cells.Conclusion: Resveratrol showed inhibitory effects on cell behaviour related to the development of endometriosis by differentially affecting growth, apoptosis, migration and stem cell phenotype of endometrial and endometriotic cells in vitro.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Reproductive Healthcare Ltd
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ENDOMETRIAL CELL PRIMARY CULTURE
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ENDOMETRIOSIS
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GENE EXPRESSION
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NATURAL COMPOUNDS
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RESVERATROL
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STEM CELLS
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PATOGENESIS
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TRATAMIENTO
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Otras Medicina Básica
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
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Patología
dc.subject.classification
Medicina Básica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2022-10-05T14:31:35Z
dc.journal.volume
44
dc.journal.number
6
dc.journal.pagination
976-990
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Madanes, Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Meresman, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Valla, Sofía Aylén. Universidad Nacional del Noroeste de la Provincia de Buenos Aires; Argentina
dc.description.fil
Fil: Hassan, Nourhan. Westfälische Wilhelms-Universität Münster ; Alemania. Cairo University; Egipto
dc.description.fil
Fil: Kiesel, Ludwig. Westfälische Wilhelms-Universität Münster ; Alemania
dc.description.fil
Fil: Greve, Burkhard. Westfälische Wilhelms-Universität Münster ; Alemania
dc.description.fil
Fil: Barañao, Rosa Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.description.fil
Fil: Götte, Martin. Westfälische Wilhelms-Universität Münster ; Alemania
dc.description.fil
Fil: Ricci, Analía Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología y Medicina Experimental. Fundación de Instituto de Biología y Medicina Experimental. Instituto de Biología y Medicina Experimental; Argentina
dc.journal.title
Reproductive Biomedicine Online
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.rbmojournal.com/article/S1472-6483(22)00091-8/fulltext
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.rbmo.2022.02.008
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