Artículo
Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis
Madanes, Daniela
; Meresman, Gabriela Fabiana
; Valla, Sofía Aylén
; Hassan, Nourhan; Kiesel, Ludwig; Greve, Burkhard; Barañao, Rosa Ines
; Götte, Martin; Ricci, Analía Gabriela
Fecha de publicación:
2022
Editorial:
Reproductive Healthcare Ltd
Revista:
Reproductive Biomedicine Online
ISSN:
1472-6483
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Research question: Does resveratrol exert a potent inhibitory effect on the development of endometriosis by interfering with some pivotal processes?Design: In-vitro cultures of primary endometriotic stromal cells, immortalized endometrial stromal (St-T1b) and endometriotic epithelial (12Z) cells were used to assess the effects of resveratrol on endometrial cell mechanisms. The effects of resveratrol on 12Z and St-T1b cell viability were assessed by MTT assay, apoptosis by FITC Annexin V assay and cleaved caspase-3 levels and cell migration by wound healing assay. The effect of resveratrol on the expression of genes related to cell migration, angiogenesis and cell stemness was evaluated by qRT-PCR.Results: Resveratrol significantly decreased cell viability (P= 0.0065 to P = 0.0180), cell migration (P < 0.001 to P = 0.0225) and increased the number of apoptotic cells (P = 0.0031 to P = 0.0432) in both cell lines. In cell lines and primary culture, the treatment reduced MMP-2/TIMP-1 (P < 0.001 to P = 0.0180), VEGF (P = 0.0052 to P = 0.0243) and Ang-1 mRNA (P < 0.001 to P = 0.0382) expression. Among the stem cell phenotype markers, resveratrol 100 µM increased mRNA expression levels of Notch-1 (P < 0.001 to P = 0.0018), KLF-4 (P = 0.0011 to P = 0.0137), SOX-2 (P < 0.001 to P = 0.0070) and TERT (P < 0.001 to P = 0.0193) in both cell lines and primary cultures. The mRNA expression level of Snail-1 increased in the cell lines (P < 0.001 to P = 0.0087), whereas OCT-4 mRNA expression increased in St-T1b (P = 0.0396) and primary cultures (P = 0.0148). Vimentin mRNA expression showed a significant upregulation in primary cultures (P < 0.001). The expression of Msi-1 (P = 0.0145) and NANOG (P = 0.0080) decreased only in St-T1b cells.Conclusion: Resveratrol showed inhibitory effects on cell behaviour related to the development of endometriosis by differentially affecting growth, apoptosis, migration and stem cell phenotype of endometrial and endometriotic cells in vitro.
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Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Citación
Madanes, Daniela; Meresman, Gabriela Fabiana; Valla, Sofía Aylén; Hassan, Nourhan; Kiesel, Ludwig; et al.; Resveratrol impairs cellular mechanisms associated with the pathogenesis of endometriosis; Reproductive Healthcare Ltd; Reproductive Biomedicine Online; 44; 6; 2022; 976-990
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