Production of laccases from agricultural wastes: Strain isolation and selection, enzymatic profiling, and lab-scale production

Abstract

Fungal laccases (copper-containing oxidases) have profound applications in bioremediation and various other industrial and biotechnological areas. This chapter outlines recent developments in laccase production technologies, with a focus on the selection of the most promising producing fungal species, general considerations on media components and culture conditions, lab-scale production, and partial purification of enzymes. Furthermore, protocols related to solid-state fermentation (utilizing agriculture wastes), such as one-step affinity chromatography (employing a mesoporous material as support), and electrophoresis (used to check the enzyme purity), are described.