Artículo
Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response. Objective(s): The hamster carcinogenesis model recapitulates oral oncogenesis. Dimethylbenz[a]anthracene (DMBA) cancerization induces early severe mucositis, affecting animal's welfare and causing tissue loss and pouch shortening. “Short” pouches cannot be everted for local irradiation for boron neutron capture therapy (BNCT). Our aim was to optimize the DMBA classical cancerization protocol to avoid severe mucositis, without affecting tumor development. We evaluated BNCT in animals cancerized with this novel protocol. Materials and methods: We studied: Classical cancerization protocol (24 applications) and Classical with two interruptions (completed at the end of the cancerization protocol). BNCT mediated by boronophenylalanine (BPA) was performed in both groups. Results: The twice-interrupted group exhibited a significantly lower percentage of animals with severe mucositis versus the non-interrupted group (17% versus 71%) and a significantly higher incidence of long pouches (100% versus 53%). Tumor development and the histologic characteristics of tumor and precancerous tissue were not affected by the interruptions. For both groups, overall tumor response was more than 80%, with a similar incidence of BNCT-induced severe mucositis. Conclusion(s): The twice-interrupted protocol reduced severe mucositis during cancerization without affecting tumor development. This favored the animal's welfare and reduced the number of animals to be cancerized for our studies, without affecting BNCT response.
Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
Título:
Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy
Santa Cruz, Iara Sofía; Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea
; Trivillin, Verónica Andrea
; Itoiz, María Elina
; Itoiz, María Elina
; Pozzi, Emiliano César Cayetano; Pozzi, Emiliano César Cayetano; Thorp, Silvia Inés; Thorp, Silvia Inés; Curotto, Paula; Curotto, Paula; Guidobono, Juan Santiago
; Guidobono, Juan Santiago
; Heber, Elisa Mercedes; Heber, Elisa Mercedes; Nigg, David W.; Nigg, David W.; Schwint, Amanda Elena
; Schwint, Amanda Elena
; Monti Hughes, Andrea
; Monti Hughes, Andrea
Fecha de publicación:
09/2020
09/2020
09/2020
Editorial:
Wiley Blackwell Publishing, Inc
Wiley Blackwell Publishing, Inc
Wiley Blackwell Publishing, Inc
Revista:
Oral Diseases
Oral Diseases
Oral Diseases
ISSN:
1354-523X
1354-523X
1354-523X
e-ISSN:
1601-0825
1601-0825
1601-0825
Idioma:
Inglés
Inglés
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IEGEBA)
Articulos de INSTITUTO DE ECOLOGIA, GENETICA Y EVOLUCION DE BS. AS
Articulos de INSTITUTO DE ECOLOGIA, GENETICA Y EVOLUCION DE BS. AS
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27
Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27
Santa Cruz, Iara Sofía; Garabalino, Marcela Alejandra; Trivillin, Verónica Andrea; Itoiz, María Elina; Pozzi, Emiliano César Cayetano; et al.; Optimization of the classical oral cancerization protocol in hamster to study oral cancer therapy; Wiley Blackwell Publishing, Inc; Oral Diseases; 26; 6; 9-2020; 1-27
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