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dc.contributor.author Moiola, Cristian Pablo
dc.contributor.author de Luca, Paola
dc.contributor.author Gardner, Kevin
dc.contributor.author Vazquez, Elba Susana
dc.contributor.author de Siervi, Adriana
dc.date.available 2017-05-16T20:21:50Z
dc.date.issued 2010
dc.identifier.citation Moiola, Cristian Pablo; de Luca, Paola; Gardner, Kevin; Vazquez, Elba Susana; de Siervi, Adriana; Cyclin T1 overexpression induces malignant transformation and tumor growth; Landes Bioscience; Cell Cycle; 9; 15; -1-2010; 3119-3126
dc.identifier.issn 1538-4101
dc.identifier.uri http://hdl.handle.net/11336/16551
dc.description.abstract Human PTEFb is a protein kinase composed by CDK9 and Cyclin T that controls the elongation phase of RNA Pol II. This complex also affects the activation and differentiation program of lymphoid cells. In this study we found that several head and neck tumor cell lines overexpress PTE Fb. We also established that Cyclin T1 is able to induce transformation in vitro, as we determined by foci and colony formation assays. Nu/nu mice s.c. injected with stable transfected Cyclin T1 cells (NIH 3T3 Cyclin T1) developed tumors faster than animals injected with control cells (NIH 3T3 β-gal). In vitro, NIH 3T3 Cyclin T1 cells show increased proliferation and CDK4-Rb phosphorylation. Even more, silencing E2F1 expression (shRNA E2F1) in NIH 3T3 cells resulted in a dramatic inhibition of Cyclin T1-induced foci. All these data demonstrate for the first time the Cyclin T1 oncogenic function and suggest a role for this protein in controlling cell cycle probably via Rb/E2F1 pathway.
dc.format application/pdf
dc.language.iso eng
dc.publisher Landes Bioscience
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject CYCLIN T1
dc.subject CDK9
dc.subject PTEFb
dc.subject.classification Bioquímica y Biología Molecular
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title Cyclin T1 overexpression induces malignant transformation and tumor growth
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-05-15T21:08:31Z
dc.identifier.eissn 1551-4005
dc.journal.volume 9
dc.journal.number 15
dc.journal.pagination 3119-3126
dc.journal.pais Estados Unidos
dc.journal.ciudad Austin
dc.description.fil Fil: Moiola, Cristian Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: de Luca, Paola. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: Gardner, Kevin. National Institutes of Health; Estados Unidos
dc.description.fil Fil: Vazquez, Elba Susana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil Fil: de Siervi, Adriana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. National Institutes of Health; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title Cell Cycle
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.4161/cc.9.15.12526
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/abs/10.4161/cc.9.15.12526
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3040930/


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info:eu-repo/semantics/restrictedAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)