Artículo
Cyclin T1 overexpression induces malignant transformation and tumor growth
Fecha de publicación:
2010
Editorial:
Landes Bioscience
Revista:
Cell Cycle
ISSN:
1538-4101
e-ISSN:
1551-4005
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Human PTEFb is a protein kinase composed by CDK9 and Cyclin T that controls the elongation phase of RNA Pol II. This complex also affects the activation and differentiation program of lymphoid cells. In this study we found that several head and neck tumor cell lines overexpress PTE Fb. We also established that Cyclin T1 is able to induce transformation in vitro, as we determined by foci and colony formation assays. Nu/nu mice s.c. injected with stable transfected Cyclin T1 cells (NIH 3T3 Cyclin T1) developed tumors faster than animals injected with control cells (NIH 3T3 β-gal). In vitro, NIH 3T3 Cyclin T1 cells show increased proliferation and CDK4-Rb phosphorylation. Even more, silencing E2F1 expression (shRNA E2F1) in NIH 3T3 cells resulted in a dramatic inhibition of Cyclin T1-induced foci. All these data demonstrate for the first time the Cyclin T1 oncogenic function and suggest a role for this protein in controlling cell cycle probably via Rb/E2F1 pathway.
Palabras clave:
Cyclin T1
,
Cdk9
,
Ptefb
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(OCA CIUDAD UNIVERSITARIA)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Citación
Moiola, Cristian Pablo; de Luca, Paola; Gardner, Kevin; Vazquez, Elba Susana; de Siervi, Adriana; Cyclin T1 overexpression induces malignant transformation and tumor growth; Landes Bioscience; Cell Cycle; 9; 15; 2010; 3119-3126
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