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dc.contributor.author
Foresi, Noelia Pamela
dc.contributor.author
Correa Aragunde, Maria Natalia
dc.contributor.author
Parisi, Gustavo Daniel
dc.contributor.author
Caló, Gonzalo Federico
dc.contributor.author
Salerno, Graciela Lidia
dc.contributor.author
Lamattina, Lorenzo
dc.date.available
2017-05-11T18:31:25Z
dc.date.issued
2010-11
dc.identifier.citation
Foresi, Noelia Pamela; Correa Aragunde, Maria Natalia; Parisi, Gustavo Daniel; Caló, Gonzalo Federico; Salerno, Graciela Lidia; et al.; Characterization of a Nitric Oxide Synthase from the Plant Kingdom: NO Generation from the Green Alga Ostreococcus tauri Is Light Irradiance and Growth Phase Dependent; American Society Of Plant Biologist; Plant Cell; 22; 11; 11-2010; 3816-3830
dc.identifier.issn
1040-4651
dc.identifier.uri
http://hdl.handle.net/11336/16316
dc.description.abstract
The search for a nitric oxide synthase (NOS) sequence in the plant kingdom yielded two sequences from the recently published genomes of two green algae species of the Ostreococcus genus, O. tauri and O. lucimarinus. In this study, we characterized the sequence, protein structure, phylogeny, biochemistry, and expression of NOS from O. tauri. The amino acid sequence of O. tauri NOS was found to be 45% similar to that of human NOS. Folding assignment methods showed that O. tauri NOS can fold as the human endothelial NOS isoform. Phylogenetic analysis revealed that O. tauri NOS clusters together with putative NOS sequences of a Synechoccocus sp strain and Physarum polycephalum. This cluster appears as an outgroup of NOS representatives from metazoa. Purified recombinant O. tauri NOS has a Km for the substrate L-Arg of 12 ± 5 μM. Escherichia coli cells expressing recombinant O. tauri NOS have increased levels of NO and cell viability. O. tauri cultures in the exponential growth phase produce 3-fold more NOS-dependent NO than do those in the stationary phase. In O. tauri, NO production increases in high intensity light irradiation and upon addition of L-Arg, suggesting a link between NOS activity and microalgal physiology.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society Of Plant Biologist
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Ostreococcus Tauri
dc.subject
Nos Sythase
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Characterization of a Nitric Oxide Synthase from the Plant Kingdom: NO Generation from the Green Alga Ostreococcus tauri Is Light Irradiance and Growth Phase Dependent
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-25T13:27:03Z
dc.identifier.eissn
1532-298X
dc.journal.volume
22
dc.journal.number
11
dc.journal.pagination
3816-3830
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Foresi, Noelia Pamela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones Biologicas; Argentina
dc.description.fil
Fil: Correa Aragunde, Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones Biologicas; Argentina
dc.description.fil
Fil: Parisi, Gustavo Daniel. Universidad Nacional de Quilmes; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Caló, Gonzalo Federico. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Salerno, Graciela Lidia. Fundación para Investigaciones Biológicas Aplicadas. Centro de Estudios de Biodiversidad y Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Lamattina, Lorenzo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Cs.exactas y Naturales. Instituto de Investigaciones Biologicas; Argentina
dc.journal.title
Plant Cell
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1105/tpc.109.073510
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.plantcell.org/content/22/11/3816
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3015112/
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