Artículo
Characterization of a Nitric Oxide Synthase from the Plant Kingdom: NO Generation from the Green Alga Ostreococcus tauri Is Light Irradiance and Growth Phase Dependent
Foresi, Noelia Pamela
; Correa Aragunde, Maria Natalia
; Parisi, Gustavo Daniel
; Caló, Gonzalo Federico
; Salerno, Graciela Lidia
; Lamattina, Lorenzo
Fecha de publicación:
11/2010
Editorial:
American Society Of Plant Biologist
Revista:
Plant Cell
ISSN:
1040-4651
e-ISSN:
1532-298X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The search for a nitric oxide synthase (NOS) sequence in the plant kingdom yielded two sequences from the recently published genomes of two green algae species of the Ostreococcus genus, O. tauri and O. lucimarinus. In this study, we characterized the sequence, protein structure, phylogeny, biochemistry, and expression of NOS from O. tauri. The amino acid sequence of O. tauri NOS was found to be 45% similar to that of human NOS. Folding assignment methods showed that O. tauri NOS can fold as the human endothelial NOS isoform. Phylogenetic analysis revealed that O. tauri NOS clusters together with putative NOS sequences of a Synechoccocus sp strain and Physarum polycephalum. This cluster appears as an outgroup of NOS representatives from metazoa. Purified recombinant O. tauri NOS has a Km for the substrate L-Arg of 12 ± 5 μM. Escherichia coli cells expressing recombinant O. tauri NOS have increased levels of NO and cell viability. O. tauri cultures in the exponential growth phase produce 3-fold more NOS-dependent NO than do those in the stationary phase. In O. tauri, NO production increases in high intensity light irradiation and upon addition of L-Arg, suggesting a link between NOS activity and microalgal physiology.
Palabras clave:
Ostreococcus Tauri
,
Nos Sythase
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Identificadores
Colecciones
Articulos(INBA)
Articulos de INST.DE INVEST. EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Articulos de INST.DE INVEST. EN BIOCIENCIAS AGRICOLAS Y AMBIENTALES
Articulos(OCA PQUE. CENTENARIO)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Citación
Foresi, Noelia Pamela; Correa Aragunde, Maria Natalia; Parisi, Gustavo Daniel; Caló, Gonzalo Federico; Salerno, Graciela Lidia; et al.; Characterization of a Nitric Oxide Synthase from the Plant Kingdom: NO Generation from the Green Alga Ostreococcus tauri Is Light Irradiance and Growth Phase Dependent; American Society Of Plant Biologist; Plant Cell; 22; 11; 11-2010; 3816-3830
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