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dc.contributor.author
Angel Villegas, Natalia
dc.contributor.author
Baronetti, Jose Luis
dc.contributor.author
Albesa, Inés
dc.contributor.author
Polifroni, Rosana
dc.contributor.author
Parma, Alberto Ernesto
dc.contributor.author
Etcheverría, Analía Inés
dc.contributor.author
Becerra, María Cecilia
dc.contributor.author
Padola, Nora Lía
dc.contributor.author
Paraje, Maria Gabriela
dc.date.available
2015-08-11T14:05:54Z
dc.date.issued
2013-12
dc.identifier.citation
Angel Villegas, Natalia; Baronetti, Jose Luis; Albesa, Inés; Polifroni, Rosana; Parma, Alberto Ernesto; et al.; Relevance of biofilms in the pathogenesis of Shiga-toxin-producing Escherichia coli infection; Hindawi Publishing Corporation; Scientific World Journal, The; ID607258; 12-2013; 1-7
dc.identifier.issn
1537-744X
dc.identifier.uri
http://hdl.handle.net/11336/1594
dc.description.abstract
The present study was designed to determine the relationships among biofilm formation, cellular stress and release of Shiga toxin (Stx) by three different clinical Shiga toxin-producing Escherichia coli (STEC) strains. The biofilm formation was determined using crystal violet stain in tryptic soy broth or thioglycollate medium with the addition of sugars (glucose or mannose) or hydrogen peroxide. The reactive oxygen species (ROSs) were detected by the reduction of nitro blue tetrazolium and reactive nitrogen intermediates (RNI) determined by the Griess assay. In addition, the activities of two antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied. For the cytotoxicity studies, Vero cells were cultured with Stx released of STEC biofilms. The addition of sugars in both culture mediums resulted in an increase in biofilm biomass, with a decrease in ROS and RNI production, low levels of SOD and CAT activity, and minimal cytotoxic effects. However, under stressful conditions, an important increase in the antioxidant enzyme activity and high level of Stx production were observed. The disturbance in the prooxidantantioxidant balance and its effect on the production and release of Stx evaluated under different conditions of biofilm formation may contribute to a better understanding of the relevance of biofilms in the pathogenesis of STEC infection.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Hindawi Publishing Corporation
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
.
dc.subject.classification
Biología Celular, Microbiología
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Relevance of biofilms in the pathogenesis of Shiga-toxin-producing Escherichia coli infection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2016-03-30 10:35:44.97925-03
dc.journal.number
ID607258
dc.journal.pagination
1-7
dc.journal.pais
Estados Unidos
dc.journal.ciudad
New York
dc.description.fil
Fil: Angel Villegas, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina;
dc.description.fil
Fil: Baronetti, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas.Centro Científico Tecnológico - CONICET - Cordoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina;
dc.description.fil
Fil: Albesa, Inés. Universidad Nacional de Cordoba. Facultad de Cs.Quimicas; Argentina;
dc.description.fil
Fil: Polifroni, Rosana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Nacional Patagonico; Argentina;
dc.description.fil
Fil: Parma, Alberto Ernesto. Universidad Nacional del Centro de la Provincia de Bs.as.. Facultad de Cs.veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Inmunoquimica y Biotecnologia; Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina;
dc.description.fil
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina;
dc.description.fil
Fil: Becerra, María Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina;
dc.description.fil
Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - CONICET - Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina;
dc.description.fil
Fil: Paraje, Maria Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico - Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina;
dc.journal.title
Scientific World Journal, The
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1155/2013/607258
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845835/?report=classic


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