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Artículo

Relevance of biofilms in the pathogenesis of Shiga-toxin-producing Escherichia coli infection

Angel Villegas, NataliaIcon ; Baronetti, Jose Luis; Albesa, Inés; Polifroni, RosanaIcon ; Parma, Alberto Ernesto; Etcheverría, Analía InésIcon ; Becerra, María CeciliaIcon ; Padola, Nora Lía; Paraje, Maria GabrielaIcon
Fecha de publicación: 12/2013
Editorial: Hindawi Publishing Corporation
Revista: Scientific World Journal, The
ISSN: 1537-744X
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Celular, Microbiología

Resumen

The present study was designed to determine the relationships among biofilm formation, cellular stress and release of Shiga toxin (Stx) by three different clinical Shiga toxin-producing Escherichia coli (STEC) strains. The biofilm formation was determined using crystal violet stain in tryptic soy broth or thioglycollate medium with the addition of sugars (glucose or mannose) or hydrogen peroxide. The reactive oxygen species (ROSs) were detected by the reduction of nitro blue tetrazolium and reactive nitrogen intermediates (RNI) determined by the Griess assay. In addition, the activities of two antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT), were studied. For the cytotoxicity studies, Vero cells were cultured with Stx released of STEC biofilms. The addition of sugars in both culture mediums resulted in an increase in biofilm biomass, with a decrease in ROS and RNI production, low levels of SOD and CAT activity, and minimal cytotoxic effects. However, under stressful conditions, an important increase in the antioxidant enzyme activity and high level of Stx production were observed. The disturbance in the prooxidantantioxidant balance and its effect on the production and release of Stx evaluated under different conditions of biofilm formation may contribute to a better understanding of the relevance of biofilms in the pathogenesis of STEC infection.
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/1594
DOI: http://dx.doi.org/10.1155/2013/607258
URL: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3845835/?report=classic
Colecciones
Articulos(CIVETAN)
Articulos de CENTRO DE INVESTIGACION VETERINARIA DE TANDIL
Articulos(IMBIV)
Articulos de INST.MULTIDISCIPL.DE BIOLOGIA VEGETAL (P)
Articulos(CCT-CENPAT)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - CENPAT
Citación
Angel Villegas, Natalia; Baronetti, Jose Luis; Albesa, Inés; Polifroni, Rosana; Parma, Alberto Ernesto; et al.; Relevance of biofilms in the pathogenesis of Shiga-toxin-producing Escherichia coli infection; Hindawi Publishing Corporation; Scientific World Journal, The; ID607258; 12-2013; 1-7
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