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dc.contributor.author
Bevacqua, Romina Jimena
dc.contributor.author
Fernández y Martín, Rafael
dc.contributor.author
Salamone, Daniel Felipe
dc.date.available
2017-05-02T19:02:01Z
dc.date.issued
2011-04
dc.identifier.citation
Bevacqua, Romina Jimena; Fernández y Martín, Rafael; Salamone, Daniel Felipe; Bovine parthenogenotes produced by inhibition of first or second polar bodies emission; Inst Histol Embriol-conicet; Biocell; 35; 1; 4-2011; 1-7
dc.identifier.issn
0327-9545
dc.identifier.uri
http://hdl.handle.net/11336/15879
dc.description.abstract
Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Inst Histol Embriol-conicet
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Oocyte Maturation
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Meiosis
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Parthenogenesis
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Fisiología
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Medicina Básica
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Bovine parthenogenotes produced by inhibition of first or second polar bodies emission
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-04-24T20:43:03Z
dc.journal.volume
35
dc.journal.number
1
dc.journal.pagination
1-7
dc.journal.pais
Argentina
dc.journal.ciudad
Mendoza
dc.description.fil
Fil: Bevacqua, Romina Jimena. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Fernández y Martín, Rafael. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Salamone, Daniel Felipe. Universidad de Buenos Aires. Facultad de Agronomía. Pabellón de Zootecnica. Laboratorio de Biotecnología Animal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Biocell
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/jhnxpn
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