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Artículo

Bovine parthenogenotes produced by inhibition of first or second polar bodies emission

Bevacqua, Romina JimenaIcon ; Fernández y Martín, RafaelIcon ; Salamone, Daniel FelipeIcon
Fecha de publicación: 04/2011
Editorial: Inst Histol Embriol-conicet
Revista: Biocell
ISSN: 0327-9545
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Fisiología

Resumen

Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes (77%) were arrested at a meiotic stage characterized by the presence of a large internal metaphase plate and absence of polar body. In experiment 2, development of embryos exposed to cytochalasin B during in vitro maturation (CytoB-IVM) or during activation (CytoB-ACT) was compared. Developmental rates did not differ between diploidization strategies, even when three agents were employed to induce activation. Both groups, CytoB-IVM and CytoB-ACT, tended to maintain diploidy. CytoB-IVM parthenogenesis could help to obtain embryos with a higher degree of homology to the oocyte donor.
Palabras clave: Oocyte Maturation , Meiosis , Parthenogenesis
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/15879
URL: http://ref.scielo.org/jhnxpn
Colecciones
Articulos(OCA PQUE. CENTENARIO)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA PQUE. CENTENARIO
Citación
Bevacqua, Romina Jimena; Fernández y Martín, Rafael; Salamone, Daniel Felipe; Bovine parthenogenotes produced by inhibition of first or second polar bodies emission; Inst Histol Embriol-conicet; Biocell; 35; 1; 4-2011; 1-7
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